Human hepatocellular carcinoma cell BEL-7402 SCID mouse model was established.Transfection was performed using lipofectamine,and flow cytometry was employed to detect TCRVβ7.1 gene expression.Caudal vein injection,subcutaneous injection adjacent to tumor and intraperitoneal injection were used to transfect the TCRVβ7.1 gene.Distribution of lymphocyte was detected by immunohistochemistry.The weight and size of tumour were measured,and inhibition rate of tumour was analyzed to study the anti-tumour effect of TCRVβ7.1 gene on SCID mice.This study provides experimental data for the anti-tumour effect of the gene in clinical treatment.
李志杰, 李贵生*. TCR基因修饰淋巴细胞体内抗肿瘤作用的研究[J]. , 2012, 31(2): 197-201.
LI Zhi-jie, LI Gui-sheng. Study of anti-tumor effect of TCR gene-modified cells. , 2012, 31(2): 197-201.
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