This paper makes a research on the technical conditions about how to abstract cellulase from Helix pomatia pomatia. The optimum conditions were studied through orthogonal experiment. The results manifest that the best abstraction pH and temperature were 5.0 and 0℃ respectively, and the optimum time is about 10 hours. On the basis of the above conditions we abstracted primary product of cellulase through partition chromatography and found the most active unity of cellulase is 1.402 U·ml-1.
孙祖莉, 刘玉田. 蜗牛纤维素酶提取工艺的初步研究[J]. , 2002, 21(4): 327-329.
Sun Zuli, Liu Yutan. A preliminary studies on the technology of cellulase extracted from Helix pomatia. , 2002, 21(4): 327-329.