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| Cloning of the microcystin-detoxifizyme gene in the main Chinese freshwater fishes |
| LIAO Wan-Qin, LIANG Xu-Fang, WANG Lin, FANG Ling, LEI La-Mei, HAN Bo-Ping |
| College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
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Abstract Microsystin-detoxifizyme gene plays an important role in microcystin detoxication in freshwater fishes. cDNA fragments that encoding partial sequences of microsystin-detoxifizyme gene were successfully amplified by RT-PCR using degenerate primers from the liver of the main Chinese freshwater fishes, including silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia. Homologous analysis show that the deduced amino acid sequences of the microsystin-detoxifizyme gene in silver carp, bighead carp, grass carp, goldfish, Chinese perch and Tilapia were 50%-60% homologus to the glutathione S-transferase gene of mammals, such as human, mouse, rat, cow, pig and sheep. The homologous analysis results indicate a big difference between the microcystin-detoxifizyme gene of freshwater fishes and the glutathione S-transferase gene of mammals, which may explain the special function of this gene in freshwater fishes for microcystin detoxication.
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Received: 25 February 2005
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