邻苯二甲酸二丁酯(DBP)胁迫对三疣梭子蟹亚急性毒性

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Abstract

Effects of dibutyl phthalate (DBP) with different concentrations on related enzyme activities and tissue of Portunus trituberculatus were researched. Crabs were sampled at 0, 5, 10, 15 and 20 d after stressing by three different concentrations. The results showed that the mortality increased with the increasing of DBP concentration. In 0.03 mg/L DBP treatment, SOD CAT and AKP activities showed an alternate fluctuation of increase and decrease. POD activity was lower than the control group at 10 d (P<0.05) and was equivalent to the control group in other times. However, ACP activity was significantly higher than the control group at 5 d (P<0.05) and maintained the level of the control group until the end of the experiment. Nevertheless, SOD activity was first increased and then showed a downward trend while the concentration was 0.2 mg/L. Although POD activity almost remained at the same level with the control group, CAT activity first decreased and then increased, and finally restored to same level of control group. ACP activity increased to the highest value in 15 d and was equal to the control group in other times. However, AKP activity first raised then recovered, showing a fluctuating trend. When the concentration was 0.4 mg/L, SOD, CAT and AKP activities showed a trend of first increasing and then decreasing. POD and ACP activities were lower than those in the control group in 20 d (P<0.05), but were equivalent to the control group in other times. Furthermore, histological examination indicated that the hepatopancreas and gill of P. trituberculatus were damaged by DBP. In addition, the higher the concentration was and longer the stress time was, the more obvious the damage was.

Key words： dibutyl phthalate (DBP) Portunus trituberculatus superoxide dismutase(SOD) catalase(CAT) tissue slices

Received: 25 August 2013

Q958.116

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	Articles by authors
	HU Dan-dan
	WANG Chun-lin
	SONG Wei-wei
	MU Chang-kao
	LIU Shuai
	LI Rong-hua

Cite this article:

HU Dan-dan,WANG Chun-lin,SONG Wei-wei, et al. The subacute toxicity of dibutyl phthalate (DBP) stress on Portunus trituberculatus[J]. , 2013, 32(4): 453-461.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I4/453

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