裂片石莼水提物清除ABTS和DPPH自由基的光谱学研究

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Abstract

The antioxidant activities of Ulva lacyuca L. aqueous extracts against DPPH and ABTS free radicals were evaluated using spectrometric methods. The results showed that the detection wavelength and stable time for ABTS system were 734 nm and 6 min respectively, while those for DPPH system were 515 nm and 30 min, respectively. The extracts effectively and rapidly removed ABTS and DPPH free radicals, and showed a dose- and time-dependent manner, indicating the potent antioxidant activities under both hydrophilic and hydrophobic conditions. Under the optimized systems, the IC₅₀ values for Se-PPC, PPC and VC against ABTS free radical were 76.40, 54.11 and 60.69 μg/ml, respectively. Se-PPC exhibited significantly higher activities than the VC. For DPPH free radicals, the IC₅₀values of Se-PPC, PPC and VC were 123.29, 76.83 and 24.79 μg/ml, respectively. Our results suggest selenium-enrichment cultured U. lacyuca L. has potential value of applications to be functional food with antioxidation.

Key words： Ulva lacyuca L. aqueous extracts antioxidant activity ABTS DPPH

Received: 25 August 2013

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	Articles by authors
	ZHONG Yu
	CHEN Tian-feng
	ZHENG Wen-jie
	YANG Yu-feng

Cite this article:

ZHONG Yu,CHEN Tian-feng,ZHENG Wen-jie, et al. Spectrometric investigation of DPPH and ABTS free radical-scavenging of Ulva lacyuca L. aqueous extracts[J]. , 2013, 32(4): 447-452.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I4/447

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