微囊藻毒素去毒酶转基因模型的构建

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Abstract

cDNA fragment encoding complete sequence of silver carp(Hypophthalmichthys molitrix) microcystin-deto-xifizyme was subcloned to construct a fusing gene expression vector pEGFP-N1-sGST,and a dicistronic expression vector pIRES2-EGFP-sGST.Using lipofectin method,the fusing gene expression vector pEGFP-N1-sGST was transfected into Hela cells.The emission of fluorescence was observed after 60 hours.Through microinjection,the dicistronic expression vector pIRES2-EGFP-sGST was delivered into embryos of zebrafish(Danio rerio),and the silver carp microcystin-detoxifizyme transgenic zebrafish was obtained.The success of the two transgenic models makes it possible to further analyze the cis-regulatory elements of the microcystin-detoxifizyme gene,and to develop a strain of transgenic planktivorous fish,used as a high-efficient biodetoxicator for microcystin in lakes and reservoirs.

Key words： Microcystin-detoxifizyme gene Microcystin Transgenic model Regulatory element Bio-detoxicator

Received: 25 February 2006

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	Articles by authors
	LIAO Wan-qin
	LIANG Xu-fang
	WANG Lin
	HAN Bo-ping

Cite this article:

LIAO Wan-qin,LIANG Xu-fang,WANG Lin, et al. Construction of microsystin-detoxifizyme transgenic model[J]. , 2006, 25(1): 25-27.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2006/V25/I1/25

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