Complete sequences of mitochondrial cytochrome b gene of Siniperca chuatsi,S.kneri,S.scherzeri,S.undulata,S.obscura,Coreosiniperca roulei,Coreoperca whiteheadii were sequenced.Combined with available homologous sequences of C.whiteheadi,C.kawamebari and C.herzi and outgroup taxa from GenBank,23 mitochondrial sequences of 17 individuals from 9 species of sinipercine fishes and 6 non-sinipercine species were analyzed to infer phylogenetic relationships among sinipercine fishes.Sequence analysis showed that the genetic distances among Siniperca species,which were from 0.015 to 0.093,were obviously smaller than those of Coreoperca species,which ranged from 0.152 to 0.178.Kimura 2-parameter model based Neighbor-Joining trees showed that sinipercine fishes consisted of a group which were further divided into two clades.Of which one clade included C.roulei and Siniperca,and another were consisted of Coreoperca,supporting the taxonomic treatment that sinipercine fishes could be divided into Siniperca and Coreoperca.Resultant phylogenetic results confirmed the very close relationship between S.chuatsi and S.kneri,showed relatively close relationship between S.scherzeri and S.undulata,and suggested that C.roulei was relatively distantly related to other Siniperca species.The relationships among the three subgroup S.chuatsi-S.kneri,S.scherzeri-S.undulata and S.obscura were not fully resolved yet in this study.In the genus Coreoperca,C.whiteheadii was distantly related to C.kawamebari,and phylogentic position of C.herzi was uncertain.Monophyly and phylogenetic positions of sinipercine fishes remained to be further studied.