微囊藻毒素对斑马鱼重要细胞因子表达的影响

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Abstract

Microcystins are hepatotoxic,causing necrosis and pooling of blood in the liver of fish.In this paper,RT-PCR was used to study the effects caused by microcystins on several important cytokines of zebra fish such as interleukin-1β(IL-1β),IL-8,IL-10,interferon(IFN),tumor necrosis factor(TNF),CXC and CXCa.Sixty zebra fish were divided into experimental group and the control,thirty in each group.The experimental group was cultivated outdoor exposing to microcystins,and the control cultivated indoor with water replacement daily.The results showed that:1) after 25 days,IFN and TNF in the liver of experimental group could be detected; 2) after 35 days,IL-10 in the gill of experimental group,CXCa in the liver of experimental group,IL-1β,TNF,IL-10,CXC and IFN in the liver of both groups were observed,and the expressions of IL-1β,TNF,IL-10 in experimental fish were higher than those of the control; 3) after 45 days,IFN in the gill and liver of experimental group,CXC in the gill of experimental group,IL-10 in gill,intestines,kidney and muscle of control group,IL-10 in liver,egg,kidney and muscle of experimental group,IL-1β in liver,egg,kidney and muscle of both groups were expressed,but expressions of IL-1β in experimental group were higher than those of the controls.In conclusion,these results suggested that the inflammation occurred in liver and gill of experimental group.

Key words： Microcystins Danio rerio cytokines RT-PCR

Received: 25 December 2006

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	Articles by authors
	TONG Fang
	ZHONG Jian-xiang
	LIANG Hui
	HUANG Jia-wei
	CHEN Meng-zhang
	CHEN Yue-fang

Cite this article:

TONG Fang,ZHONG Jian-xiang,LIANG Hui, et al. Influences of Microcystins on several significant cytokines of zebra fish,Danio rerio[J]. , 2006, 25(6): 545-549.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2006/V25/I6/545

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