湛江等鞭金藻对抗生素的反应及无菌化培养

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Abstract

Effects of 5 kinds of antibiotics (chloramphenicol, ampicillin, streptomycin, kanamycin, gentamycin) at different concentrations on the growth of Isochrysis zhangjiangensis were studied, the axenic culture was obtained by mixed antibiotics. The results showed that:(1) There were differences among different antibiotics, chloramphenicol was the most susceptible antibiotic, the inhibition rate was 27.02% at the concentration of 50 μg·mL^-1, more than 50 μg·mL^-1 the inhibition rate was 80%. I.zhangjiangensis was not susceptible to ampicillin, streptomycin, kanamycin and gentamycin, there was stimulation at the lower concentration of the last three antibiotics. (2) Except for ampicillin at the concentration of 50 μg·mL^-1, there were bacteriostasis effects of the other treatments. (3) Mixed antibiotics (500 μg·mL^-1streptomycin,1000 μg·mL^-1 kanamycin, and 50 μg·mL^-1 gentamycin) were added to the culture medium by 4 combinations, the axenic culture was obtained, and there were stimulation on the growth of I.zhangjiangensis.

Key words： Isochrysis zhangjiangensis Antibiotics Axenic culture

Received: 25 April 2007

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	Articles by authors
	HUANG Zhen-hua
	LIU Xiao-juan
	HU Zhang-xi
	DUAN Shun-shan

Cite this article:

HUANG Zhen-hua,LIU Xiao-juan,HU Zhang-xi, et al. Effects of antibiotics on the growth of Isochrysis zhangjiangensis and axenic culture[J]. , 2007, 26(2): 120-121.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2007/V26/I2/120

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