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| Primary study on tissue culture of Chondria crassicaulis |
| WU Xun-jian, HE Li-hong, SONG Yi, SHEN Song-dong |
| Life Science College, Soochow University, Suzhou City, 215123 |
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Abstract Regeneration polarity of Chondria crassicaulis was studied in solid medium. We used three different liquid medium and three different temperatures to explore the effects of some external factors to the cultivation of chopped tissues of Chondria crassicaulis. We found that under artificial culture environment there was no obvious polarity in the regeneration of fragments. When cultured in PES, modified ASP1, modified ES culture media and under 10℃, 15℃, 22℃, it was found that the formations of buds were abundant and best in the segments under 15℃ in modified ES liquid media. We also found that there were two kinds of sporangia, cruciform and four conical, in the cultivation, and tetraspore of Chondria crassicaulis could develop into four kinds of seedlings.
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Received: 25 August 2007
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| [1] 黄宗国主编.1994.中国海洋生物种类与分布[M].北京,海洋出版社:15-233
[2] Edmonds J S,Shibata Y,Yang F Q,et al.1997.Isolation and synthesis of 1-Deoxy-1-dimethy-larsinoylribitol-5-sulfate,a natural constituent of Chondria crassicaulis and other red algae[J].Tetrahedron Letters,38 (33),5819-5820.
[3] Harada H.1997.Selective Cytotoxicity of Marine Algae Extracts of Several Human Leukemic Cell Lines[J].Cytotechnology,25(1-3):213-219.
[4] 殷丽明,魏云莉,丁伟.1988.青岛沿海常见海藻抗肿瘤及抗菌活性初报[J].中国海洋药物,7(1/2):39-41.
[5] 王素娟主编.1994.海藻生物技术[M].上海:上海科学技术出版,20-21.
[6] 尤丽莉.2005.龙须菜切段再生、原生质体分离培养及果孢子与四分孢子的收集和培养的研究[D].青岛:中国科学院青岛海洋研究所.
[7] 陈昌生,章景荣.1987.项群藻切断离体培养[J].福建水产,1:44-49.
[8] 王素娟,徐志东,陈昌生.1985.细弱红翎菜髓部组织培养与切段再生[J].海洋渔业,4:155-159.
[9] 李伟新,朱仲嘉,刘凤贤编.1982.海藻学概论[M].上海:上海科学技术出版社.
[10] 骆其君,裴鲁青,费志清.1993.不同世代大石花菜切段育苗的生态试验[J].东海海洋,11(2):62-66. |
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