锯缘青蟹(Scylla serrata)Hsp70基因cDNA的克隆及序列分析

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Abstract

Heat shock protein Hsp70 gene of Scylla serrata was cloned by RACE and RT-PCR.The full length cDNA of hsp70 obtained 2482 b,containing an ORF of 1950 bp,encoding 649 amino acid residues with estimated molecular weight of 71.06 kD and theoretical isoelectric point of 5.24,a 3'UTR of 158 bp,a 5'UTR of 40 bp.By the analyse of antheprot,two signature sequences of HSP70 family: IFDLGGGTFDVSIL,IVLVGGSTRIPKIQK,Dnak sequence: DLGTT-S-V,the non-organellar motif: RARFEEL,bipartite nuclear localization signal: KKDPSESKRALRRL,and C-terminal four amino acids of Hsp70: EEVD were detected in the predicted amino acid sequence.Through blast analysis,the ORF of the Hsp70 gene sequence of Scylla serrata has 84.02% identity with the Hsp70 cDNA sequence of Litopenaeus vannamei,and 83.87% identity with Penaeus monodon,and 79.60% identity with Macrobrachium rosenbergii.Under the alignment of Hsp70 amino acids deduced from DNA sequences,there is 92.17% identity between Scylla serrata and Penaeus monodon,and 92.64% identity between Scylla serrata and Macrobrachium rosenbergii,and 96.47% identity between Scylla serrata and Macrobrachium rosenbergii.

Key words： Hsp70 gene RT-PCR RACE Scylla serrata

Received: 25 February 2008

Q915.819+.6

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	Articles by authors
	YUAN Jia-en
	XU Zhong-neng
	HAN Bo-ping
	KONG Yi-jun
	LEI La-mei
	XIE Shu-tao

Cite this article:

YUAN Jia-en,XU Zhong-neng,HAN Bo-ping, et al. RACE clone Hsp70 gene ORF of Scylla serrata and sequence analysis[J]. , 2008, 27(1): 24-30.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2008/V27/I1/24

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