近江牡蛎HSP70基因对溶藻弧菌感染的反应

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Abstract

In order to investigate the role of HSP70 of oyster(Crassostrea hongkongensis) against bacterial infection,we established a real-time,quantitative fluorescent RT-PCR method to examine the expression patterns of HSP70 mRNA in mantle,digestive gland,gills,muscle,heart and haemocytes of the oyster responding to Vibiro alginolyticus challenge.The results showed that after the oysters were injected with live V.alginolyticus,HSP70 mRNA expression in gills,mantle and haemocytes was up-regulated at first,peaked at 3 hr,6 hr and 24 hr,respectively,then gradually decreased to the control level at 12 hr,24 hr sand 72 hr,respectively.The phenomenon was defined as time-dependent expression pattern of HSP70 gene.Meanwhile,the HSP70 mRNA levels in heart and digestive gland significantly increased and reached the highest values at 3 hr and 6 hr,respectively,and then dropped rapidly.But in muscle,the HSP70 mRNA expression did not increased upon the bacterial challenge during the entire experiment period.The up-regulated mRNA expression of HSP70 induced by bacterial challenge suggested an important role for HSP70 gene in immune response in oysters.

Key words： Crassostrea hongkongensis HSP70 gene Vibiro alginolyticus real-time fluorescence quantitative RT-PCR mRNA expression

Received: 25 June 2011

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	CHEN Chen
	ZHANG Qi-zhong
	ZHANG Zhan-hui

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CHEN Chen,ZHANG Qi-zhong,ZHANG Zhan-hui. mRNA Expression of Heat Shock protein 70 gene in the oyster(Crassostrea hongkongensis) responding to Vibiro alginolyticus challenge[J]. , 2011, 30(3): 295-300.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I3/295

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