转基因甘蔗对根际土壤微生物多样性的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (502 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

Effect of chitinase and β-1,3- glucanase transgenic sugarcane on microbial diversity in rhizosphere soil was studied by Biolog plates method. The results showed that the Average Well Color Development(AWCD) of transgenic sugarcane was higher than non-transgenic sugarcane, indicating the carbon utilization capability of microbial community was higher. The indices of Shannon(H), Smipson(1/D) and Mcintosh(U) were calculated respectively to show the richness, dominance and evenness of the functional diversity accordingly. The results showed that the rhizosphere soil microbial diversity indices of transgenic sugarcane were all higher than those of non-transgenic sugarcane;the dominance of the functional diversity was remarkably improved, followed by the evenness;the difference of the richness was not significant. It indicated that the root exudates of transgenic sugarcane in the growing season had stimulated the growth of rhizosphere soil microbial, which might have induced the development of the microbial community with certain physical characteristics. This preliminary study showed transgenic sugarcane might affect the microbial community composition in rhizosphere soil.

Key words： transgenic sugarcane soil microbial microbial diversity

Received: 25 June 2010

:	S154
	Q145

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	TAN Yan-hua
	PENG Cun-zhi
	ZHANG Shu-zhen
	WANG Dong-mei
	LIU En-ping

Cite this article:

TAN Yan-hua,PENG Cun-zhi,ZHANG Shu-zhen, et al. Effect of transgenic sugarcane on microbial community diversity in rhizosphere soil[J]. , 2010, 29(3): 242-246.

URL:

http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I3/242

[1] 王振,赵廷昌,刘学敏,邓欣.2007.转基因作物对土壤微生物多样性影响[J].植物保护,33(4):15-20.

[2] 李长林,张欣,吴建波,刘惠芬.2008.转基因棉花对根际土壤微生物多样性的影响[J].农业环境科学学报,27(5):1857-1859.

[3] McGregor A N,Tumer M A.2000.Soil effects of transgenic agriculture:biological processes and ecological consequences[J].NZ Soil News,48(6):166-169.

[4] Finlay B J,Maberly S C,Cooper J I.1997.Microbial diversity and ecological function[J].Oikos,80:209-213.

[5] Groffman P M,Bohlen P J.1999.Soil and sediment biodiversity-cross-system comparisons and large-scale effects[J].Biological Science,49:139-148.

[6] Bruinsma M,G A Kowalchuk,J A van Veen.2003.Effects of genetically modified plants on microbial communities and processes in soil[J].Biology and Fertility of Soils,37:329-337.

[7] 王建武,冯远娇,骆世明.2002.转基因作物对土壤生态系统的影响[J].应用生态学报,13(4):491-494.

[8] 蔡燕飞,廖宗文.土壤微生物生态学研究方法进展[J].土壤与环境,2002,11(2):167-171.

[9] Preston-Mafham J,Boddy L,Randerson P F.2002.Analysis of microbial community functional diversity using sole-carbon-source utilization profiles-a critique[J].FEMS Microbiology Ecology,42:1-14.

[10] Garland J L,Mills A L.1991.Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J].Applied Environmental Microbiology,57(8):2351-2359.

[11] 席劲瑛,胡洪营,钱易.Biolog方法在环境微生物群落研究中的应用[J].微生物学报,2003,43(1):138-141.

[12] 顾丽红,张树珍,杨本鹏,蔡文伟,黄东杰,王文治,李娇.2008.几丁质酶和β-1,3-葡聚糖酶基因导入甘蔗[J].分子植物育种,6(2):277-280.

[13] Schutter M,Dick R.2001.Shift in substrate utilization potential and structure of soil microbial communities in response to carbon substrates[J].Soil Biology and Biochemistry,33:1481-1491.

[14] Hacker C A,Griffiths B S.1997.Statistical analysis of the time-course of Biolog substrate utilization[J].Journal of Microbiological Methods,30:63-69.

[15] 罗希茜,郝晓晖,陈涛,邓婵娟,吴金水,胡荣桂.2009.长期不同施肥对稻田土壤微生物群落功能多样性的影响[J].生态学报,29(2):740-748.

[16] Benizri E,Amiaud B.2005.Relationship between plants and soil microbial communities in fertilized grasslands[J].Soil Biology and Biochemistry,37(11):2055-2064.

[17] Zabinski C A,Gannon J E.1997.Effects of recreational impacts on soil microbial communities[J].Environtal Management,21(2):233-238.

[18] Zak J C,Willig M R,Moorhead D L,Wildman H G.1994.Functional diversity of microbial communities a quantitative approach[J].Soil Biology & Biochemistry,26:1101-1108.

[19] 杨永华,姚健,华晓梅.2000.农药污染对土壤微生物群落功能多样性的影响[J].微生物学杂志,20(2):23-25.

[20] Milling A,Smalla K,Franz X M,Schloter M,Munch J.2005.Effects of transgenic potatoes with an altered starch composition on the diversity of soil and rhizosphere bacteria and fungi[J].Plant and Soil,266(12):23-39.

[21] Brusetti L,Francia P,Bertolini C,Pagliuca A,Borin S.2005.Bacterial communities associated with the rhizosphere of transgenic Bt 176 maize(Zea mays)and its non-transgenic counterpart[J].Plant and Soil,266(12):11-21.

[22] Tesfayea M,Dufault N S,Dornbusch M R,Allan D L,Vance C P,Samac D A.2003.Influence of enhanced malate dehydrogenase expression by alfalfa on diversity of rhizobacteria and soil nutrient availability[J].Soil Biology & Biochemistry,35:1103-1113.

[23] Schmalenberger A,Tebbe C C.2003.Genetic profiling of noncultivated bacteria from the rhizospheres of sugar beet (Beta vulgaris) reveal field and annual variability but no effect of transgenic herbicide resistance[J].Canadian Journal of Microbiology,49:1-8.

[24] Sessitsch A,Kan F Y,Pfeifer U.2003.Diversity and community structure of culturable Bacillus spp.populations in the rhizospheres of transgenic potatoes expressing the lytic peptide cecropin B[J].Applied Environmental Microbiology,22:149-158.

[25] Lottmann J,Heuer H,Vries J,Mahn A,Düring K,Wackernagel W,Smalla K,Berg G.2000.Establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community[J].FEMS Microbiology Ecology,33(1):41-49.

[26] Oger P,Petit A,Dessaux Y.1997.Genetically engineered plants producing opines alter their biological environment[J].Nature Biotechnology,15(4):369-372.

[27] Saxena D,Stotzky G.2001.Bacillus thuringiensis (Bt)toxin released from root exudates and biomass of Bt corn has no apparent effect on erathworns,nematodes,protozoa,bacteria,and fungi in soil[J].Soil Biology and Biochemistry,33(9):1255-1230.

[28] Kaare Johnsen,Carsten S Jacobsen,Vigdis Torsvik.2001.Pesticide effects on bacterial diversity in agricultural soils--a review[J].Biology and Fertility of Soils,33:443-453.

[29] Garland J L,Mills A L.1994.A community-level physiological approach for study in gmicrobial communities[A].In:Ritz K,Dighton J,Giller K E.eds.Beyond the Biomass:Composition and Functional Analysis of Soil Microbial Communities.London:Wiley-Sayce Publications,77-83.

[30] 郑华,欧阳志云,方治国,赵同谦.2004.BIOLOG在土壤微生物群落功能多样性研究中的应用[J].土壤学报,41(3):456-461.

[1]	. [J]. Ecological Science, 2020, 39(1): 213-.