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Analyses of sequence variation and secondary structure on rDNA ITS regions of the strains of redtide-caused harmful algae Phaeocystis globosa
LIU Hai-lin1,2, ZHANG Qun1, JANG Qi-ming1, MA Ben1
1. Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China; 2. Floricultural Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China
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Abstract  

Sequences of rDNA ITS region in the Strains P1,P2 and ZhJ1 of Phaeocystis globosa collected from Hongkong and Zhanjiang in South China Sea were determined.Combined with 13 homologous sequences downloaded from GenBank,the aligned ITS sequences of Phaeocystis(including 5.8srDNA) were 904bp,of which 271 sites were variable,and 221 sites were parsimony-informative,the contents of(A+T)(34.5%) were less than those of(G+C)(65.4%).20 sites were variable and no parsimony-informative sites were found in the sequences of P1,P2 and ZhJ1,sequence similarities were 97.9%-98.5%.The resolution to interspecific and intraspecific relationships in ITS sequences were better than 18s,28s gene.The topologies of phylogenetic trees derived from Neighbor-Joining memod,Maximum parsimony method and Bayesian inference were consistent.Different Phaeocystis speciesclustered independently.Although different geographical strains of the same Phaeocystis species might intertwine together,the strains of the same geographical origin strains tended to cluster.A comparison of secondary structures of rDNA ITS sequences revealed that the 5.8srDNA structures were basically the same in different species of the same genus, indicating the specificity of the genus;The secondary structure of the ITS1,2 regions were much different among species;indicating that the secondary structure of rDNA ITS region could provide some molecular structure information for the classification of Phaeocystis.
Key words： Phaeocystis      rDNA ITS region      secondary structure      sequence analysis      coastal waters of China     
Received: 25 October 2010     
:  Q178.532  
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LIU Hai-lin
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LIU Hai-lin,ZHANG Qun,JANG Qi-ming. Analyses of sequence variation and secondary structure on rDNA ITS regions of the strains of redtide-caused harmful algae Phaeocystis globosa[J]. , 2010, 29(5): 432-437.
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