萘胁迫下秋茄MnSOD基因和C4H基因的实时定量表达分析

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Abstract

The expression of C₄H gene and MnSOD gene in leaf,stem and root of Kandelia candel responded to the naphthalene(NAP) was evaluated in this study.Real-time PCR analysis demonstrated that the regulation patterns in three tissues were different.The expression of C₄H gene in leaf and stem was higher than that in root.The expression level of MnSOD was in the order of leaf >stem>root.The expression level of C₄H and MnSOD increased with the enhancement of NAP.A significantly positive correlation was also found between NAP and C₄H expression in leaf,and the correlation coefficient was 0.846 and 0.902(p<0.05),respectively.However,there was no obvious correlation between NAP and C₄H expression in stem or root.The results indicated that the C₄H and MnSOD gene regulation systems were the most sensitive in leaf,and the expression analysis indicated that they were good indicators for NAP stress.

Key words： Kandelia.candel real-time PCR naphthalene C4H MnSOD

Received: 25 April 2012

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	SONG Hui
	WANG You-Shao

Cite this article:

SONG Hui,WANG You-Shao. Expression analysis of MnSOD gene and C4H gene in Kandelia candel under naphthalene stress[J]. , 2012, 31(2): 104-108.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2012/V31/I2/104

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