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Construction of petroleum-degrading bacterial consortium from mangrove sediments
LI Mei, LIAO Bao-wen, CHEN Yu-jun
Research Institute of Tropical Forestry, CAF, Guangzhou 510520, China
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Abstract  

In recent years,marine pollution has been increasing at global level,and the petroleum pollution is particularly serious.The technology of microbial degradation has become one of effective ways to solve marine petroleum pollution.Mangrove ecosystem plays an important role in accumulating and degrading petroleum residues in the coastal areas.In order to construct the petroleum-degrading bacterial consortium,with alkanes or naphthalene as the sole carbon source,two alkane degrading strains(named Z1,Z3)and two aromatic hydrocarbon-degrading strains(named N1,N4)were isolated by the enrichment culture method(ECM)from the petroleum contaminated mangrove sediments.Using the orthogonal experiments of three factors and three levels,the optimized strain combination was obtained,and the optimum dosages of each strain were determined.The results showed that a combination of two alkane-degrading strains Z1,Z3 and one aromatic-degrading strain N1 got the highest degradation rate of 47.3%,with the initial petroleum-hydrocarbon concentration of 2.0 g·L-1,the inoculation amount of 6%,the culture temperature of 30℃,and the aerobic culture time of 72 h.When N1,Z1 and Z3 dosing with the ratio of 3:1:3,the strain combination got the highest degradation rate of 51.2% after 72 h aerobic culture.

Key wordsmangrove wetlands      petroleum-degrading bacterial consortium construction      optimum dosage ratio     
Received: 25 October 2012     
:  S796  
  S714  
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LI Mei
LIAO Bao-wen
CHEN Yu-jun
Cite this article:   
LI Mei,LIAO Bao-wen,CHEN Yu-jun. Construction of petroleum-degrading bacterial consortium from mangrove sediments[J]. , 2012, 31(5): 553-557.
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http://www.ecolsci.com/EN/     OR     http://www.ecolsci.com/EN/Y2012/V31/I5/553
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