红树林湿地石油降解菌群的构建

Abstract
Figure/Table
References
Related Citation (2)

Download: PDF (238 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

In recent years,marine pollution has been increasing at global level,and the petroleum pollution is particularly serious.The technology of microbial degradation has become one of effective ways to solve marine petroleum pollution.Mangrove ecosystem plays an important role in accumulating and degrading petroleum residues in the coastal areas.In order to construct the petroleum-degrading bacterial consortium,with alkanes or naphthalene as the sole carbon source,two alkane degrading strains(named Z1,Z3)and two aromatic hydrocarbon-degrading strains(named N1,N4)were isolated by the enrichment culture method(ECM)from the petroleum contaminated mangrove sediments.Using the orthogonal experiments of three factors and three levels,the optimized strain combination was obtained,and the optimum dosages of each strain were determined.The results showed that a combination of two alkane-degrading strains Z1,Z3 and one aromatic-degrading strain N1 got the highest degradation rate of 47.3%,with the initial petroleum-hydrocarbon concentration of 2.0 g·L^-1,the inoculation amount of 6%,the culture temperature of 30℃,and the aerobic culture time of 72 h.When N1,Z1 and Z3 dosing with the ratio of 3:1:3,the strain combination got the highest degradation rate of 51.2% after 72 h aerobic culture.

Key words： mangrove wetlands petroleum-degrading bacterial consortium construction optimum dosage ratio

Received: 25 October 2012

:	S796
	S714

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Mei
	LIAO Bao-wen
	CHEN Yu-jun

Cite this article:

LI Mei,LIAO Bao-wen,CHEN Yu-jun. Construction of petroleum-degrading bacterial consortium from mangrove sediments[J]. , 2012, 31(5): 553-557.

URL:

http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2012/V31/I5/553

[1] 赵晟,洪华生,张珞平,陈伟琪.中国红树林生态系统服务的能值价值[J].资源科学,2007,29(1):147-154.
[2] 林鹏,陈荣华.红树林有机碎屑在河口生态系统中的作用[J].生态学杂志,1991,10(2):45-48.
[3] 骆苑蓉,胡忠,郑天凌,黄栩.红树林沉积物中的微生物对苯并[a] 芘的降解研究[J].厦门大学学(自然科学版),2005,44(S1):75-79.
[4] 李玫,廖宝文,章金鸿.底泥微生物在红树林生态系统中的作用[J].广州环境科学,2006,04:21-25.
[5] 陆俊锟,康丽华,陈俊,吕成群,黄宝灵,江业根.华南三地红树林土壤微生物及其与土壤化学性质的相关性研究[J].林业科学研究,2008,21(4):523-527.
[6] 龙寒,向伟,庄铁城,林鹏.红树林区微生物资源[J].生态学杂志,2005,24(6):696-702.
[7] 陈碧娥,刘祖同.湄洲湾海洋细菌降解石油烃研究[J].石油学报,2001,17(5):31–35.
[8] 张景来,王剑波,常冠钦.环境生物技术及应用[M].北京:化学工业出版社,2002.232-483.
[9] 丁明宇,黄建,李永祺.海洋微生物降解石油的研究[J].环境科学学报,2001,21(1):84-88.
[10] Cortf B C.Effect of bioremediation agents on oil biodegradation in medium-fine sand Applied Bioremediation of petroleum Hydrocarbons[J].Battelle Press,1995,6(3):423-434.
[11] 马文漪,杨柳燕.环境微生物工程[M].南京:南京大学出版社,1998.258-263.
[12] 涂书新,韦朝阳.我国生物修复技术的现状与展望[J].地理科学进展,2004,23(6):20-32.
[13] 郑天凌,庄铁城,蔡立哲,田蕴,郭楚玲,徐美珠,李少菁.微生物在海洋污染环境中的生物修复作用[J].厦门大学学报自然科学版),2001,40(2):524-534.
[14] 田胜艳,刘宪斌,高绣花.石油污染海洋环境中的细菌群落[J].环境与可持续发展,2006,4(1):32-35.
[15] 邵宗泽,许晔,马迎飞,郭庆.2株海洋石油降解细菌的降解能力[J].环境科学,2004,25(5):133-137.
[16] 谢丹平,尹华,彭辉,叶锦韶,张娜.混合菌对石油的降解[J].应用与环境生物学报,2004,10(2):210-214.
[17] 谢重阁.环境中石油污染物的分析技术[M].北京:中国环境科学出版社,1987.1-10.
[18] 刘陈立,邵宗泽.海洋石油降解微生物的分离鉴定[J].海洋学报,2005,27(4):114-120.
[19] 庄铁诚,林鹏.红树林下土壤微生物对柴油的降解[J].厦门大学学报自然科学版,1995,34(3):442-446.
[20] 邱金泉,王静,张辉,张雨山.海洋中多环芳烃生物降解与修复研究进展[J].化学工业与工程,2010,02:117-121.
[21] Hozumi T,Tsutsumi H,Kono M.Bioremediation on the shore after an oil spill from theNakhodka in the Sea of Japan.Ⅰ.Chemistry and characteristics of heavy oil loaded on the Nakhodka and biodegradation tests by a bioremediation agent with microbiological cultures in the laboratory[J].Marine Pollution Bulletin,2000,40(4): 308-314.