不同盐度和Fe3+浓度对小球藻生长、硝酸还原酶活性及基因表达的影响

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Abstract

The effects of four different salinity and five iron concentrations on the growth,nitrate reductase(NR)activity and its expression were investigated in Chlorella pyrenoidosa F-9-3,which was selected after ultraviolet radiation.The salinity experiment showed that C.pyrenoidosa F-9-3 grew faster in 0-0.30mol·L^-1 NaCl medium than in 0.45 mol·L^-1 NaCl medium.And NR activity and its expression were higher in 0.15 and 0.30mol·L^-1 NaCl groups than those of other two groups.The iron experiment showed that the growth,NR activity and gene expression of C.pyrenoidosa F-9-3 were all higher in 0.03 and 0.06 mmol·L^-1 Fe³⁺ treatments than those in other Fe³⁺ concentration mediums.The growth was inhibited in 0.12 mmol·L^-1 iron medium and NR expression was the highest in iron deficiency group.It can be concluded that optimum culture condition of the alga was 0-0.30 mol·L^-1 NaCl and 0.03-0.06 mmol·L^-1 iron concentration.

Key words： Chlorella salt Fe3+ concentration nitrate reductase gene expression

Received: 25 August 2012

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	DU Yu
	SUN Xue
	XU Nian-jun

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DU Yu,SUN Xue,XU Nian-jun. Effects of different salinity and iron concentration on growth, nitrate reductase activity and its expression in Chlorella[J]. , 2012, 31(4): 441-445.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2012/V31/I4/441

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