3种不同食性淡水鱼类谷胱甘肽S-转移酶Pi、Mu、Theta型cDNA序列的克隆分析及表达

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Abstract

Glutathione S-transferase(GST) of freshwater fish is a kind of PhaseⅡdetoxification enzymes and is important to detoxification from fish body,by catalyzing the conjugation of GSH with toxin.In this study,the partial cDNAs encoding pi-class,mu-class,theta-class GST(GSTpi,GSTmu,GSTtheta) were cloned and sequenced from the grass carp(Ctenopharyngodon idella) and the Nile tilapia (Oreochromis nilotica),and the GSTmu,GSTtheta were cloned and sequenced from the sliver carp(Hypophthalmichthys molitrix) by RT-PCR and RACE method.Relative amino acids were deduced.The phylogenetic tree constructed on the basis of GST sequences showed that the GSTs amino acid sequences of sliver carp,grass carp and nile tilapia were highly conserved in fish,which had lower homology with that of amphibian,bird and human.This finding was consistent with the special function of these genes in freshwater fish in toxin detoxification.In the different kinds of fishes,the homology of GSTtheta is lower than GSTpi and GSTmu,it seems to be conserved with different tolerance for toxin in different fishes.Meanwhile,the constitutive expression of liver GSTs genes were determined by real-time RT-PCR.The mRNA expression level of Nile tilapia GSTs genes were the lowest,GSTtheta expression was significantly lower than that of grass carp(P<0.05),GSTmu expression was significantly lower than that of sliver carp(P<0.05).This study helps to understand the functions of different classes of glutathione S-transferases(GSTs) genes in the metabolism of toxics in limnetic fishes.

Key words： grass carp sliver carp Nile tilapia glutathione S-transferase molecular cloning sequence analysis expression

Received: 25 August 2010

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	Articles by authors
	YAO Yu
	LIANG Xu-fang
	WANG Lin
	LUAN Tian
	LIU Li-man

Cite this article:

YAO Yu,LIANG Xu-fang,WANG Lin, et al. Cloning, sequence analysis and expression of Pi、Mu and Theta classes of glutathione S-transferases genes in three freshwater fishes[J]. , 2010, 29(4): 324-331.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I4/324

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