Regeneration polarity of Chondria crassicaulis was studied in solid medium. We used three different liquid medium and three different temperatures to explore the effects of some external factors to the cultivation of chopped tissues of Chondria crassicaulis. We found that under artificial culture environment there was no obvious polarity in the regeneration of fragments. When cultured in PES, modified ASP1, modified ES culture media and under 10℃, 15℃, 22℃, it was found that the formations of buds were abundant and best in the segments under 15℃ in modified ES liquid media. We also found that there were two kinds of sporangia, cruciform and four conical, in the cultivation, and tetraspore of Chondria crassicaulis could develop into four kinds of seedlings.
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