Effects of environmental factors on the vitality of sperm in csiaenops ocellatus
WEI Ping1, YE Ting1, WU Xiang-dan2, YAN Jia-qiang1, ZHU Jun-quan1
1. Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo 315211, China; 2. Library of Ningbo University, Ningbo 315211, China
The effects of pH value, salinity, glucose and ions solutions on the sperm vitality of Csiaenops ocellatus were investigated by measuring the activation ration, moving time and life-span of sperm.The results indicated that the average density of sperm was (1.203±0.22)×1010·ml-1 and the suitable salinity for sperm activation and movement was 20-35, while pH value 6.0～8.5.The activation rate, moving time and life-ban of sperm reached to 94.32%, 9.14 min and 12.55 min respectively when the sperm was activated in salinity 25.The proper concentration of KCl, NaCl, CaCl2 solutions for sperm activation and movement was 600～700 mmol·L-1, 600 mmol·L-1 and 400～600 mmol·L-1 respectively, while the proper concentration of glucose solution was 700～900mmol·L-1.No significant differences were found in the activation rate when the sperm was activated by the artificial seawater lacking of HCO3-, Mg2+ or Ca2+, but the moving time and survival time of sperm became shorter.
