Effects of feeding frequencies on behaviors and immune function of Litopenaeus vannamei (Boone)
YU He-nan1,2, LIN Xiao-tao2, XU Zhong-neng2, ZHOU Xiao-zhuang3
1. Guang Dong Province Ocean Engineering Vocational School, Guangzhou 510320, China; 2. Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou 510632, China; 3. Guang Dong Province Ocean and Fishery Nature Reserve Management Office, Guangzhou 510222, China
The experiment was conducted for 30 days in order to determine the effects of different feeding frequencies (1, 2 and 6 times per day) on behaviors, growth and non-special immunity factors of Litopenaeus vannamei. The specific growth rate, movement time and movement distances increased progressively as feeding frequency increased. In daytime, the movement frequencies in the group fed for 6 times per day were significantly higher than those in other groups (p<0.05).However, at night, the movement frequencies in the group fed for 2 times per day were significantly higher than those in other groups (p<0.05). When feeding 1 time per day, the activities of phenoloxidase (PO), catalase(CAT) and superoxide dismutase (SOD) of the shrimp declined, but malondialdehyde (MDA) level increased. During the experiment, the values of PO, CAT, SOD and MDA of the shrimps fed 2 times per day did not change obviously, but they grew slowly when fed 6 times per day.
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