低温驯化和外源糖对爪哇伪枝藻冷胁迫的影响

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摘要

低温是影响荒漠藻类生存的重要环境因子.研究了低温驯化和外源糖对爪哇伪枝藻冷胁迫的作用.实验测定了低温和外源糖处理下伪枝藻某些生理活性指标的变化.结果表明,低温胁迫导致伪枝藻生物量和光合活性的显著降低,低温驯化和外源糖在一定程度上对藻细胞叶绿素α含量和光合活性具有促进作用,低温驯化明显提高了细胞内可溶性蛋白的含量,而对丙二醛的含量影响不大.研究还发现,低温驯化明显降低了细胞膜的相对透性,而外源糖对细胞膜的相对透性影响不大.此外,低温驯化促进了伪枝藻胞外多糖的大量合成.研究结果初步证实了低温驯化和外源糖类对于提高伪枝藻的冷胁迫耐受性具有调节作用.

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	饶本强
	吴沛沛
	李敦海
	陈坤
	刘永定

关键词 ：荒漠藻, 爪哇伪枝藻, 低温胁迫, 低温驯化, 外源糖, 冷胁迫耐受性

Abstract：

Desert algae have a broad distribution in terrestrial environments,and the algae have been proved to be able to well adapt to extreme environments.Low temperature or cold stress is one of important ecological factors influencing survival of desert algae.In this study,a typical cyanobacteria species,Scytonema javanicum(Kütz.)Born et Flah,was isolated from desert algal crusts and selected as research material.In the experiments,2℃,10℃ and 28℃(as control)were selected for temperature treatments,and simultaneously low temperature domestication and exterior sugars such as sucrose and glucose were used to treat the algal cultures under different low temperatures.The results showed that low temperatures resulted in an obvious decrease in biomass and PS Ⅱ activity in S.javanicum. Low temperature domestication and exterior sugars promoted chlorophyll a content and PS Ⅱ activity of the alga to some extent,and low temperature domestication obviously increased intracellular production of water-soluble proteins;while no influence on MDA content was observed.In addition,we found that low temperature domestication evidently decreased secretion of EPS,but exterior sugars had no obvious role in relative osmotic activities of S.javanicum.The results suggest that low temperature domestication and exterior sugars may be crucial for tolerance of S.javanicum to low temperature or cold stress.

Key words： desert alga S.javanicum low temperature stress low temperature domestication exterior saccharides endurance of cold stress

收稿日期: 2011-04-25

Q948.1

基金资助:淡水生态与生物技术国家重点实验室资助项目(2008FBZ21);中国博士后科学基金资助项目(20100480920);武汉市科技局与内蒙古自治区发改委重大科技产业化专项(200720112031);河南省高校青年骨干教师资助计划项目;信阳师范学院高层次人才科研启动基金项目,信阳师范学院青年骨干教师资助计划项目

引用本文:

饶本强1,2*, 吴沛沛2, 李敦海2, 陈坤1, 刘永定2. 低温驯化和外源糖对爪哇伪枝藻冷胁迫的影响[J]. , 2011, 30(2): 128-134.
RAO Ben-qiang1,2, WU Pei-pei2, LI Dun-hai2, CHEN Kun1, LIU Yong-ding2. Influences of low temperature domestication and exterior saccharides on endurance of Scytonema javanicum to cold stress. , 2011, 30(2): 128-134.

链接本文:

http://www.ecolsci.com/CN/Y2011/V30/I2/128

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