We investigated the effects of cadmium(Cd2+) with different concentrations on the antioxidant enzyme activity of Tegillarca granosa by setting four Cd2+ gradients(0,0.025,0.05,and 0.1 mg/L) and sampling clam tissue at 12,24,48,96 and 144 h.The activities of antioxidant enzymes were investigated,including superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPX),and glutathione S-transferase(GST) in visceral mass,and gill tissue of T.granosa was as indicator of antioxidant properties.The results showed that SOD activities were significantly higher in the groups with middle(0.05 mg/L) and low(0.025 mg/L) concentrations of Cd2+ than those in control group after 12-24 h.However,there was no significant difference between high concentration(0.1 mg/L) and control group.With prolonged exposure time and enhanced Cd2+ concentration,significant inhibition was observed between high and middle concentration groups and control group during 48-144 h.The tendency of GPX and GST activity was similar to that of SOD activity,which reached the peak at 12-48 h,and then significantly decreased as compared with control group.Furthermore,CAT enzyme had no significant difference among low,middle and control groups at the 144th hour.So,it was concluded that Cd2+ could damage the antioxidant enzyme system of T.granosa by induction of ROS.
孙彬, 葛奇伟, 陆慧贤, 徐永健*. 镉对泥蚶抗氧化酶系统的影响[J]. , 2011, 30(4): 383-388.
SUN Bin, GE Qi-wei, LU Hui-xian, XU Yong-jian. Effects of cadmium exposure on antioxidant enzyme system of Tegillarca granosa. , 2011, 30(4): 383-388.
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