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大亚湾海域可培养细菌鉴定及系统发育分析
江晓亮, 王朝晖*, 王剑, 熊毅俊, 林朗聪
暨南大学水生生物研究所, 广州 510632
Identification and phylogenetic analysis of culturable bacteria in Daya Bay
JIANG Xiao-liang, WANG Zhao-hui*, WANG Jian, XIONG Yi-jun, LIN Lang-cong
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
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摘要 

对大亚湾海域表层水体的可培养细菌进行分离,测定了分离培养细菌的16S rRNA 基因V3 区序列,并采用该研究中的35 个序列与从GenBank 下载的46 个序列构建了系统树,对细菌进行了分子鉴定。在9 个站点的表层水体中共分离得到70株菌株,分属35 个种。其中α-变形菌纲(Alphaproteobacteria)和放线菌纲(Actinobacteria)为分离菌株最多的两个菌纲,分离率分别为40%和35.7%;而β-变形菌纲(Betaproteobacteria)仅分离到1 株(1.4%)。此外,还分离得到盖球菌属(Kytococcus)、间胞囊菌属(Intrasporangium)等非常见菌株。大亚湾海域细菌的Shannon-Wiener's 多样性指数为3.21。大亚湾海域表层水体可培养细菌丰富的种类多样性以及较高的α-变形菌分离率,说明了该海域具有良好的水质,同时也是潜在的微生物菌种资源库。

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江晓亮
王朝晖
王剑
熊毅俊
林朗聪
关键词 大亚湾可培养细菌16S rRNA 基因系统发育分析    
Abstract

Culturable bacteria were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and Intrasporangium, which were rarely reported, were also isolated. The value of Shannon-Wiener`s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.

Key wordsDaya Bay    culturable bacteria    16S rRNA gene    phylogenetic analysis
收稿日期: 2013-06-25     
:  983  
引用本文:   
江晓亮, 王朝晖*, 王剑, 熊毅俊, 林朗聪. 大亚湾海域可培养细菌鉴定及系统发育分析[J]. , 2013, 32(3): 290-297.
JIANG Xiao-liang, WANG Zhao-hui*, WANG Jian, XIONG Yi-jun, LIN Lang-cong. Identification and phylogenetic analysis of culturable bacteria in Daya Bay. , 2013, 32(3): 290-297.
链接本文:  
 http://www.ecolsci.com/CN/Y2013/V32/I3/290
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