三种鲫鱼品系同工酶比较研究

摘要
图/表
参考文献
相关文章 (2)

全文: PDF (1168 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

采用聚丙烯酰胺凝胶垂直板电泳技术,对彭泽鲫、银鲫D系以及野鲫三种鲫鱼品系的心、肝和肾脏组织的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、酯酶(EST)和超氧化物歧化酶(SOD)同工酶表型进行了比较研究.结果表明彭泽鲫乳酸脱氢酶同工酶比银鲫D系在肝组织多出二条酶带(LDH7'和LDH8');超氧化物歧化酶在心和肾组织中分别多出一条酶带(SOD12'),表明彭泽鲫和银鲫已在生化水平产生明显的分化,推测它们可能起源于不同的地区,由不同的祖先,独立演化而形成.此外,彭泽鲫和银鲫D系的同工酶电泳图谱都包含野鲫的基本酶带,而彭泽鲫和野鲫的酯酶同工酶电泳图谱尤为相似,推测彭泽鲫和银鲫可能起源于野鲫,而彭泽鲫和野鲫的关系较近,银鲫和野鲫的关系较远.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	崔淼
	赵俊
	陈湘粦

关键词 ：同工酶, 彭泽鲫, 银鲫, 野鲫

Abstract：

The expressions patterns of five isozymes(LDH,MDH,ME,EST and SOD) were investigated in different tissues of Pengze crucian carp(Carassius auratus),silver crucian carp D(C.auratus gibelio),and wild crucian carp(C.auratus),respectively,using polyacrylamide gel electrophoresis.Two bands(LDH7'and LDH8') of LDH isozyme in liver tissue of Pengze crucian carp were more than those of silver crucian carp D.The main differences in SOD existed at heart and kidney,which showed one more band(SOD12') of Pengze crucian carp than those of silver crucian carp D.The results indicated that Pengze crucian carp is different from sliver crucian carp in biochemical level;they perhaps come from different region and have different ancestor.Meanwhile,the electrophoretogram of Pengze crucian carp and silver crucian carp all included the basal patterns of wild crucian carp.Specially,EST isozyme patterns between Pengze crucian carp and wild crucian carp were similar among the patterns of several isozyme.It is presumed that Pengze crucian carp and silver crucian carp were all originated from wild crucian carp.The relation between Pengze crucian carp and wild crucian carp is closer than that of silver crucian carp and wild crucian carp.

Key words： isozyme Pengze crucian carp(C.auratus) silver crucian carp D(C.auratus gibelio) wild crucian carp(C.auratus)

收稿日期: 2012-04-25

:	Q951
	S91

基金资助:广东省自然科学基金(950478);广东省科技攻关计划(2002C20324);广东省教育厅科学基金(200127220121)

引用本文:

崔淼1,2, 赵俊1*, 陈湘粦1. 三种鲫鱼品系同工酶比较研究[J]. , 2012, 31(2): 155-160.
CUI Miao1,2, ZHAO Jun1, CHEN Xiang-lin1. Comparative studies on isozymes of three strains of Carassius auratus. , 2012, 31(2): 155-160.

链接本文:

http://www.ecolsci.com/CN/Y2012/V31/I2/155

[1] Markert C,M∅ller F.Multiple forms of enzymes:Tissue,ontogenetie and speeies speeific patterns[J].Proceedings of the National Academy of Sciences USA,1959,45(5):753-763.
[2] 杨兴棋,陈敏容,俞小牧,陈宏溪.江西彭泽鲫生殖方式的初步研究[J].水生生物学报,1992,16(3):277-280.
[3] 张辉,董新红,叶玉珍,吴清江.三个三倍体鲫鱼品系及野鲫mtDNA的比较研究[J].遗传学报,1998,25(4):330-336.
[4] Zhou L,Wang Y,Gui J F.Analysis of genetic heterogenetity among five gynogenetic clones of gynogenetic silver crucian carp markers[J].Cytogenetics and Cell Genetics,2000,88(1-2):133-139.
[5] 朱蓝菲,蒋一珪.银鲫种内的遗传标记及其在选种中的应用[J].水生生物学报,1987,11(2):105-111.
[6] 李名友,周莉,杨林,刘静霞,桂建芳.彭泽鲫的分子遗传分析及其与方正银鲫A系的比较[J].水产学报.2002,26(5):472-476..
[7] Shaw C R,Prasad R.Starch gel electrophoresis of enzymes:A compilation of recipes[J].Biochemical Genetics,1970,4(2):297-320.
[8] 朱蓝菲.鱼类同工酶和蛋白质的聚丙烯酰胺凝胶电泳法[J].水生生物学报,1992,16(2):183-185.
[9] 刘青华.黑龙江银鲫和鲫酯酶同工酶的比较研究[R].黑龙江水产研究所研究报告,1984,183(21):23-26.
[10] 黄民生,秦长庚,潘淑英,王修勇,李晓辉,田树魁.滇池高背鲫和方正银鲫酯酶、乳酸脱氢酶同工酶的比较研究[J].动物学研究,1988,9(1):69-73.
[11] 王蕊芳,施立明,贺维顺.不同地理区域鲫鱼染色体银染核仁组织者的比较研究[J].动物学研究,1988,9(2):165-170.
[12] 候亚义,袁传宓.异育银鲫、鲫和白鲫的肌浆蛋白及同工酶(EST、MDH)的电泳比较研究[J].南京师大学报(自然科学版),1989,12(3):69-73.
[13] 张四明,龙华,张兴忠.方正银鲫、白鲫与鲫线粒体DNA限制性内切酶酶切比较[J].水产学报,1992,16(2):120-129.
[14] 蒋一珪,梁绍昌,陈本德,俞豪祥,单仕新,杨德龙,林绥恩,沈根泉.异源精子在银鲫雌核发育子代中的生物学效应[C].水生生物学集刊,1983,8(1):1-13.
[15] 桂建芳.卵母细胞在细胞周期调控研究中贡献[J].中国科学基金,1996,10(3):173-178.
[16] 桂建芳.银鲫天然雌核发育机理研究的回顾与展望[J].中国科学基金,1997,11(1):11-16.
[17] Fan Z,Shen J.Studies on the evolution of bisexual reproduction in crucian carp(Carassius auratus gebelio Bloch) [J].Aquaculture,1990,84(3-4):235-244.
[18] 樊连春,赖宇鹏,朱蓝菲,梁绍昌,桂建芳.雌核发育银鲫两个不同品系线粒体DNA的比较[J].海洋与湖沼,2000,31(4):370-377.
[19] 朱蓝菲,蒋一珪.银鲫不同雌核发育系的生物学特性比较研究[J].水生生物学报,1993,17(2):112-120.
[20] 桂建芳,朱蓝菲,魏雪虹.雌核发育银鲫的遗传多样性及其育种意义[J].遗传,1997,19(增刊):37-38.
[21] 周莉,刘静霞,桂建芳.应用微卫星标记雌核发育银鲫的遗传多样性初探[J].动物学研究,2001,22(4):257-264.
[22] Zhou L,Wang Y,Gui J F.Molecular analysis of silver crucian carp(Carassiu auratus gibelio Bloch) clone by SCAR marker[J].Aquaculture,2001,201(3-4):219-228.