Expression analysis of MnSOD gene and C4H gene in Kandelia candel under naphthalene stress
SONG Hui, WANG You-Shao
1. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2. Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen 518121, China
The expression of C4H gene and MnSOD gene in leaf,stem and root of Kandelia candel responded to the naphthalene(NAP) was evaluated in this study.Real-time PCR analysis demonstrated that the regulation patterns in three tissues were different.The expression of C4H gene in leaf and stem was higher than that in root.The expression level of MnSOD was in the order of leaf >stem>root.The expression level of C4H and MnSOD increased with the enhancement of NAP.A significantly positive correlation was also found between NAP and C4H expression in leaf,and the correlation coefficient was 0.846 and 0.902(p<0.05),respectively.However,there was no obvious correlation between NAP and C4H expression in stem or root.The results indicated that the C4H and MnSOD gene regulation systems were the most sensitive in leaf,and the expression analysis indicated that they were good indicators for NAP stress.
宋晖, 王友绍*. 萘胁迫下秋茄MnSOD基因和C4H基因的实时定量表达分析[J]. , 2012, 31(2): 104-108.
SONG Hui, WANG You-Shao. Expression analysis of MnSOD gene and C4H gene in Kandelia candel under naphthalene stress. , 2012, 31(2): 104-108.
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