In order to investigate the biological toxicity of Sonneratia apetala,mammal rats,the international standards required toxicity tests fish(zebra fish)and the major species of mangrove mudskippers were served as recipients to observe the acute oral toxicity-fixed dose in rats.Improved Karber's method in zebra fish and mudskippers was used;semi-lethal toxicity test concentration (LC50)and 95% confidence limits calculation were employed in the study.Our results showed that in the 5 000 mg/kg body weight, Sonneratia apetala fruit and leaves could not influence rat behavior,with the 14 d mortality rate being 0,and semi-lethal dose(LD50) greater than 5 000 mg/kg body weight,suggesting they were non-toxic substances.For the toxicity to fish species,the fruit aqueous extract toxicity intensity overweighed the leaves aqueous extract toxicity to the same fish.In the same aqueous extract,the sensitivity of mudskipper was stronger than that of zebra fish,in the order of 24 hLC50 > 4 8 hLC50 >72 hLC50 > 96 hLC50 >1 000 mg·L-1, suggesting a low toxicity.Based on the findings,we conclude that Sonneratia apetala will not pose biological toxicity hazards.
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