利用原子吸收光谱法和电镜能谱仪法分析重金属Pb、Cd在水稻颖果不同部位中的分配

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摘要

比较研究了传统原子吸收光谱法与电镜能谱仪法分析重金属Pb、Cd在水稻籽粒不同部位中的分配情况,研究结果表明:两种方法均显示,Pb、Cd在水稻颖果中的分配为胚>糊粉层>果皮>近糊粉层处胚乳>颖果中心,其中,胚、糊粉层和果皮Pb、Cd含量差异较小,整个胚乳Pb、Cd含量变化也不大,但胚、糊粉层和果皮Pb、Cd含量显著高于胚乳.扫描能谱仪能够准确反映重金属在植物不同部位分布的差异,结果与原子吸收光谱法无显著差异.扫描能谱仪法的优点在于操作方便快捷,但是只能用于定性,不能定量测定出某一部位重金属的准确含量,因此,不能代替传统的原子吸收光谱法.

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	陈娟
	孙鉴坤
	方元平

关键词 ：原子吸收光谱法, 电镜能谱仪, Pb, Cd, 水稻颖果

Abstract：

Traditional atomic absorption spectroscopy method and the environmental scanning electron microscopy method are compared in analyzing the distribution of heavy metal Pb and Cd in rice grain.Two methods show that the concentrations of Pb and Cd in different parts of rice caryopsis are in the order of embryo>aleuron layer>pericarp>external endosperm>center of caryopses.Smaller difference in the content of Pb and Cd is found in embryo,aleurone layer and pericarp;besides,little difference exists in the whole endosperm,but the contents of Pb and Cd in embryo,aleurone layer and pericarp are significantly higher than in the endosperm.Environmental scanning electron microscopy can accurately reflect the difference of distribution of heavy metals in different parts of plant,and there is no obvious difference as compared to atomic absorption spectroscopy. Environmental scanning electron microscopy method is easy and quick;however,it can only detect qualitatively but can not detect accurately the content of elements in a specific part,so it can not take the place of the traditional method.

Key words： atomic absorption spectroscopy environmental scanning electron microscopy Pb Cd rice caryopsis

收稿日期: 2009-08-25

Q95

基金资助:湖北省科技厅自然科学基金项目(2007ABA364);湖北省教育厅自然科学中青年基金项目(Q200727003);黄冈师范学院博士基金(06CD179)资助

引用本文:

陈娟, 孙鉴坤, 方元平*. 利用原子吸收光谱法和电镜能谱仪法分析重金属Pb、Cd在水稻颖果不同部位中的分配[J]. , 2009, 28(4): 348-351.
CHEN Juan, SUN Jian-kun, FANG Yuan-ping. Analysis on the distribution of heavy metal Pb and Cd in rice grain by atomic absorption spectroscopy and environmental scanning electron microscopy. , 2009, 28(4): 348-351.

链接本文:

http://www.ecolsci.com/CN/Y2009/V28/I4/348

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