Traditional atomic absorption spectroscopy method and the environmental scanning electron microscopy method are compared in analyzing the distribution of heavy metal Pb and Cd in rice grain.Two methods show that the concentrations of Pb and Cd in different parts of rice caryopsis are in the order of embryo>aleuron layer>pericarp>external endosperm>center of caryopses.Smaller difference in the content of Pb and Cd is found in embryo,aleurone layer and pericarp;besides,little difference exists in the whole endosperm,but the contents of Pb and Cd in embryo,aleurone layer and pericarp are significantly higher than in the endosperm.Environmental scanning electron microscopy can accurately reflect the difference of distribution of heavy metals in different parts of plant,and there is no obvious difference as compared to atomic absorption spectroscopy. Environmental scanning electron microscopy method is easy and quick;however,it can only detect qualitatively but can not detect accurately the content of elements in a specific part,so it can not take the place of the traditional method.
陈娟, 孙鉴坤, 方元平*. 利用原子吸收光谱法和电镜能谱仪法分析重金属Pb、Cd在水稻颖果不同部位中的分配[J]. , 2009, 28(4): 348-351.
CHEN Juan, SUN Jian-kun, FANG Yuan-ping. Analysis on the distribution of heavy metal Pb and Cd in rice grain by atomic absorption spectroscopy and environmental scanning electron microscopy. , 2009, 28(4): 348-351.
[1] Liu J,Li K,Xu J,etai.2003.Interaction of Cd and five mineral nutrients for uptake and accumulation in different rice cultivats and genotypes[J].Field Crop Res,83(3):271-281.
[3] Obata H,Umebayashi M.1997.Effects of cadmium on mineral nutrient concentrations in plants differing in tolerance for cadmium[J].J Plant Nutr,20:97-105.
[4] Jackson A P,Alloway B J.1992.The transfer of cadmium from agriculture soils to the human food chain[M].In:Adriado DC (Ed).Biogeochemistty of Trace Metals.Boca Raton:Lewis Publiishers,109-158.
[7] Joyce C,Deneau A,Peterson K,et al.2005.The concentrations and distributions of phytic acid phosphorus and other mineral nutrients in wild-type and low phytic acid JS-12-LPA wheat (Triticum aestivum) grain parts[J].Can J Bot,83(12):1599-1607.
[11] WHO.1972.Evaluation of certain food additives and the contaminants mercury,lead and cadmium.Sixteenth Report of the Joint FAO/WHO Expert committee on Food Additives.Geneva[J].Tech Report Ser,505:51-59.
[12] Zhang G P,Fukami M,Seldmoto H.2000.Genotypic differences in effects of cadmium on growth and nutrient compositions wheat[J].J Plant Nutr,23(9):1337-1350.
