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不同无机碳源对粉核油球藻生长的影响
刘然1,2, 刘晓娟1, 王铭1, 徐宁1, 李爱芬1*
1. 暨南大学水生生物研究所, 广州, 510632; 2. 山东省博兴第二中学, 滨州, 256508
Effects of inorganic carbon sources on growth of Pinguiococcus pyrenoidosus CCMP 2078
LIU Ran1,2, LIU Xiao-juan1, WANG Ming1, XU Ning1, LI Ai-fen1
1. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; 2. The Second Middle School of Boxing, Binzhou 256508, China
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摘要 

研究了2种无机碳源对粉核油球藻(Pinguiococcus pyrenoidosus CCMP2078)生长的影响。结果表明,适量添加NaHCO3和通CO2P.pyrenoidosus CCMP2078的生长均有促进作用,NaHCO3的添加方式对该藻的生长具有显著差异。一次性添加和分批添加NaHCO3的优化浓度分别为5mmoL·L-1和20mmoL·L-1,后者对数生长末期的细胞密度、干重和比生长速率均最高,分别为8.93×106cells·mL-1,0.247g·L-1和0.225dL-1。通入CO2的体积分数为0.5%时,最有利于藻细胞的生长,对数生长末期的细胞密度、干重和比生长率分别为3.83×107cells·mL-1、0.42g·L-1和0.212d-1

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刘然
刘晓娟
王铭
徐宁
李爱芬
关键词 粉核油球藻无机碳源生长    
Abstract

Effects of two types of inorganic carbon sources on the growth of Pinguiococcus pyrenoidosus CCMP 2078 were investigated in this study. The results showed that the growth of P.pyrenoidosus CCMP 2078 was significantly promoted by adding appropriate amount of NaHCO3 or CO2, and there were significant differences among different additions of NaHCO3. The optimal concentrations of NaHCO3 added for one time and added equally for several times were 5 mmoL·L-1 and 20 mmoL·L-1, respectively. For the latter treatment, parameters of cell density, dry weight, and the specific growth rate reached to the highest value, which were 8.93×106cells·mL-1, 0.247 g·L-1, and 0.225 d-1, respectively. When CO2 content was 0.5%, P. pyrenoidosus CCMP 2078 reached to the highest growth rate, and parameters of cell density, dry weight, and the specific growth rate were 3.83×107cells·mL-1, 0.42 g·L-1, and 0.212 d-1, respectively.

Key wordsPinguiococcus pyrenoidosus CCMP 2078    inorganic carbon sources    growth
收稿日期: 2007-06-25     
:  Q949.2  
基金资助:广东省科技攻关项目(2005B33201001)
引用本文:   
刘然1,2, 刘晓娟1, 王铭1, 徐宁1, 李爱芬1*. 不同无机碳源对粉核油球藻生长的影响[J]. , 2007, 26(3): 227-231.
LIU Ran1,2, LIU Xiao-juan1, WANG Ming1, XU Ning1, LI Ai-fen1. Effects of inorganic carbon sources on growth of Pinguiococcus pyrenoidosus CCMP 2078. , 2007, 26(3): 227-231.
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 http://www.ecolsci.com/CN/Y2007/V26/I3/227
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