|
|
|
| Separation and characteristics of pigment-protein complexes in Siphonous Green Algae, Bryopsis corticulans |
| LIU Xiao-juan1, LI Ai-fen1, CHEN Min2, DUAN Shun-shan1 |
| 1. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; 2. Department of biochemistry, Yantai University, Yantai 264005, China |
|
|
|
|
Abstract Pigment-protein complexes of Bryopsis corticulans were separated by PAGE and sucrose gradient centrifugation, and its characteristics were also investigated. Results showed that seven pigment-protein complexes were obtained by PAGE, which were CPIa1, CPIa2, CP I, LHCP1, LHCP2, CPa, LHCP3+3, and two free pigment bands(FP)Fca、FC. Five zones were separated by improved discontinuous sucrose gradient centrifugation. It indicated that zone Ⅰ was FP;zone Ⅱ was a light-harvesting complexes of PSⅡ with lower molecular weight as LHCP3+3, zone Ⅲ was aggregated light-harvesting complexes of PS Ⅱ as LHCP1. Both zone Ⅱ and Ⅲ contained large amount of Chlb and sinphonaxanthin except Chla, suggesting that they were characteristic of sinphonaxanthin-Chla/b-protein complexes;zone Ⅳ showed only one band in PAGE, and there was shoulder peak of Chlb in absorption spectra, containing 66 and 56 kDa peptides, so it was small PS I complexes of CP I a.
|
|
Received: 25 August 2004
|
|
|
|
|
|
| [1] Anderson J M.1985.Chlorophyll-proteincomplexes of a marine green alga, Codum species(Siphonales) [J].Biochim BiophysActa, 806(11):145-153.
[2] 陈敏,李爱芬,周百成.2001.海洋管藻目绿藻刺松藻光系统Ⅰ复合物的分离[J].生物化学与生物物理进展,28(6):858-861.
[3] 陈敏,李爱芬,宫宝安.2002.管藻目绿藻刺松藻LHCⅡ复合物的分离[J].植物生理学通讯,38(1):12-16.
[4] Anderson J M.1983.Chlorophyll protein complexes of a Codium species including a light-harvesting siphonaxanthin-chlorophyll a/b protein complexes, an evolutionary relic of some Chlorophyta[J].Biochim Biophys Acta, 724:370-380.
[5] Arnon DI.1949.Copper enzymes in isolated chloroplasts pholyphenoloxidase in Beta vulgaris[J].Plant Physiol, 24:1-15.
[6] 李桐柱,林世青.1995.一个高分辨率的凝胶电泳系统及其从蓝藻中分离的叶绿素蛋白复合体[J].植物学报,37(1):34-40.
[7] Laemmli U.1970.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].Nature, 227(5259):680-685.
[8] 陈敏,李爱芬,周百成.2000.管藻目绿藻刺松藻(Codium fragile)PSⅡ捕光复合物的结构研究[J].烟台大学学报(自然科学与工程版),13(1):38-44.
[9] 陈敏,李爱芬,周百成.2003.二次电泳法分离管藻目绿藻PSI亚复合物研究[J].海洋科学,27(11):38-42. |
|
|
|
