宁波港压载水的细菌多样性研究

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Abstract

We investigated the impacts of anthropogenic activities on structure and composition of bacterial communities and evaluated how bacterial communities responded to environmental gradients at Ningbo port. Bacteria in the ballast water from Ningbo port were isolated, and the bacterio plankton diversity was studied by using the Sherlock Microbial Identification System, 16S rRNA gene PCR amplification, and restriction fragment length polymorphism. Meanwhile, 16S rRNA clone library was constructed to reveal the composition and structure of bacterial communities in the most of ballast water. The results showed that there were Alteromonas sp., Pseudoalteromonas sp., Marinobacter sp. and Roseobacter sp. in Indian Ocean, Ochrobactrum sp., Methylobacterium sp., Pseudomonas sp., Psychrobacter sp., Pseudomonas sp., Burkholderia sp. and Staphylococcus sp. in Singapore ballast water, Vibrio sp., Legionella sp., Moraxella sp. and Acinetobacter sp. in American ballast water. Ballast water contains a large number of potential pathogens.

Key words： Ningbo port ballast water 16S rRNA gene RFLP bacteria diversity

Received: 25 December 2012

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	Articles by authors
	LI Chun-li
	LIU Bing
	ZHOU Jun
	WANG Zhong-hua
	JIANG Nan
	SU Xiu-rong
	LI Tai-wu

Cite this article:

LI Chun-li,LIU Bing,ZHOU Jun, et al. Study on genetic diversity of bacteria in ballast water from Ningbo port[J]. , 2012, 31(6): 363-644.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2012/V31/I6/363

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