抗麻痹性贝毒素GTX2,3单克隆抗体的制备及特性分析

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Abstract

To obtain hybridoma cell line which can secrete anti-gonyautoxin 2,3(GTX2,3) monoclonal antibodies(mAb), the immunogen(GTX2,3-BSA) and solid phase immunogen(GTX2,3-KLH) were prepared by the method of aldehyde conjugation, in which GTX 2,3 was conjugated with BSA and KLH respectively. The spleen cells from BALB/c mice immunized with GTX2,3-BSA were fused with murine Sp2/0 myeloma cells in PEG4000. The specific hybridoma cells and the ascites were analyzed by ELISA. Three specific hybridoma cell lines which can secrete anti-GTX 2,3 mAb were obtained. The titer of the mAb in the ascites was about 1.4×10^-5. The specific anti-GTX2,3 mAb can be obtained from hybridoma successfully, which can used to detect the PSP from seafood.

Key words： GTX2 3 PSP Monoclonal antibody

Received: 25 April 2005

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	Articles by authors
	XIANG Jun-jian
	TANG Qi
	LUO Hui-wu
	JIANG Tian-jiu
	DENG Ning
	TANG Yong
	YANG Hong-yu

Cite this article:

XIANG Jun-jian,TANG Qi,LUO Hui-wu, et al. Preparation and characterization of monoclonal antibodes against Paralytic Shellfish Poisoning Toxins GTX2,3[J]. , 2005, 24(2): 132-135.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2005/V24/I2/132

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