利用调制荧光仪在线监测叶绿素荧光

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Abstract

This paper introduced an in situ monitoring technique of chlorophyll fluorescence using portable Pulse-Amplitude Modulation fiuorometer, PAM-2100.With this technique F_t, F_m', Y, rETR, qP, qN or NPQ, PAR, and leaf temperature can be monitored without disturbing plant natural photosynthesis.An in situ monitoring case with Eichhornia crassipes was examined.Chlorophyll fluorescence were measured every 5 min and monitored continuously for 225 min.The results indicated that the changes of chlorophyll fluorescence parameters depended on the variation of environmental PAR.Some parameters such as F_t, rETR, qN and NPQ showed a consistent change with PAR, while F_m', Y and qP changed reversely.This case demonstrated that in situ monitoring technique was reliable, simple, rapid and sensitive.Along with the appearance of new modulated fluorometers suit for in situ monitoring, this technique may be widely used in plant ecology research.

Key words： Chlorophyll fluorescence On-line monitoring PAM fluorometer

Received: 25 June 2005

Q945.11

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	Articles by authors
	HAN Zhi-guo
	LEI La-mei
	HAN Bo-ping

Cite this article:

HAN Zhi-guo,LEI La-mei,HAN Bo-ping. In situ monitoring of chlorophyll fluorescence using PAM fluorometer[J]. , 2005, 24(3): 246-249.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2005/V24/I3/246

[1] Schreiber U. 2004. Pulse-amplitude-modulation (PAM)fluorometry and saturation pulse method:an overview[A]. In:Papageorgiou G C, Govindjee (Eds.) ChlorophyllFluorescence:A Signature of Photosynthesis[C]. Dordrecht:Springer. 279-319.

[2] 武海,张树源,许大全,等.1997.珊瑚树叶片叶绿素荧光非光化学淬灭的日变化和季节变化[J].植物生理学报,23(2):145-150.

[3] 彭长连,林植芳,林桂珠.1998.C₃和C₄植物叶片对光氧化响应的日变化[J].热带亚热带植物学报,6(3):233-238.

[4] 温达志,叶万辉,林植芳,等.全光和遮阴下两种亚热带木本植物的光合作用对光的响应[J].热带亚热带植物学报,9(3):248-255.

[5] 冯玉龙,冯志立,曹坤芳.2001.砂仁叶片光破坏的防御[J].植物生理学报,27(6):483-488.

[6] 许晓明,陆巍,唐运来,等.2003.自然条件下杂交水稻优129的光抑制及其恢复特性[J].中国水稻科学,17(2):156-160.

[7] 高洁,曹坤芳,王焕校,等.2004.干热河谷主要造林树种光合作用光抑制的防御机制[J].应用与环境生物学报,10(3):286-291.

[8] Zhang S, Gao R. 1999. Diumal changes of gas exchange,chlorophyll fluorescence, and stomatal aperture of hybrid poplar clones subjected to midday light stress[J].Photosynthetica, 37:559-571.

[9] Martínez-Carrasco R, Sánchez-Rodriguez J, Pérez P. 2002.Changes in chlorophyll fluorescence during the course of photoperiod and in response to drought in Casuarina equisetifolia Forst. And Forst.[J]. Photosynthetica, 40:363-368.

[10] 齐欣,曹坤芳,冯玉龙.2004.热带雨林蒲桃属3个树种的幼苗光合作用对生长光强的适应[J].植物生态学报,28(1):31-38.

[11] 梁红柱,窦德泉,冯玉龙.2004.热带雨林下砂仁叶片光合作用和叶绿素荧光参数在雾凉季和雨季的日变化[J].生态学报,24(7):1421-1429.

[12] He J, Lee S K. 2004. Photosynthetic utilization of radiant energy by temperate lettuce grown under natural tropical condition with manipulation of root-zone temperature[J].Photosynthetica, 42:457-463.

[13] Bilger W, Schreiber U, Bock M. 1995. Determination of the quantum efficiency of photosystem Ⅱ and of non-photochemical quenching of chlorophyll fluorescence in the field[J]. Oecologia, 102:425-432.

[14] Lüttge U, Fetene M, Liebig M, et al. 2001. Ecophysiology of niche occupation by two giant rosette plants, Lobelia gibberoa Hemsl and Solanecio gigas (Vatke) C. Jeffrey, in an afromontane forest valley[J]. Annals of Botany, 88:267-278.

[15] Genty B, Briantais J-M, Baker N R. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochimica Biophysica Acta, 990:87-92.

[16] Van Kooten O, Snel J F H. 1990. The use of chlorophyll fluorescence nomenclature in plant stress physiology[J].Photosynthesis Research, 25:147-150.

[17] Bilger W, Bjorkman O. 1990. Role of the xanthophylls cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis[J].Photosynthesis Research, 25:173-186.

[18] Maxwell K, Johnson G N. 2000. Chlorophyll fluorescencea practical guide[J]. Journal of Experimental Botany, 51:659-668.

[19] Schreiber U, Bilger W, Neubauer C. 1994. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis[A]. In:Schulze E-D, Caldwell MM (Eds.) Ecophysiology of Photosynthesis[C]. Berlin:Springer-Verlag. 49-70.

[20] Anonymous. 2003. Portable Chlorophyll Fluorometer PAM-2100:Handbook of Operation[M]. Eichenring:Heinz Walz GmbH.

[21] Govindjee. 1995. Sixty-three years since Kautsky:chlorophyll a fluorescence induction[J]. Australia Journal of Plant Physiology, 22:131-160.

[22] Demmig-Adams B, Adams W W Ⅲ. 1992. Photoprotection and other responses of plants to high light stress[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 43:599-626.

[23] Müller P, Li X-P, Niyogi K K. 2001. Non-photochemical quenching:a response to excess light energy[J]. Plant Physiology, 125:1558-1566.

[24] Jones R J, Kildea T, Hoegh-Guldberg O. 1999. PAMchlorophyll fluorometry:a new in situ technique for stress assessment in Scleractinian corals, used to examine the effects of cyanide from cyanide fishing[J]. Marine Pollution Bulletin, 20:864-874.

[25] McMinn A, Ryan K, Gademann R. 2003. Diumal changes in photosynthesis of Antarctic fast ice algal communities determined by pulse amplitude modulation fluorometry[J].Marine Biology, 143:359-367.

[26] Torzillo G, Bernardini P, Masojidek J. 1998. On-line monitoring of chlorophyll fluorescence to assess the extent of photoinhibition of photosynthesis induced by high oxygen concentration and low temperature and its effect on the productivity of outdoor cultures of Spirulina platensis (Cyanobacteria)[J]. Journal of Phycology, 34:504-510.