微卫星位点筛选方法综述

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Abstract

Microsatellite DNA has been shown to be highly polymorphic and co dominant and therefore quite useful as a good genetic marker.However,the wide utilization of microsatellite marker is hindered by the difficulty of obtaining microsatellite loci from interesting species.Recently,many methods have been developed for the isolation of microsatellite loci.Among these methods,database searching is the most convenient method for obtaining new microsatellite loci,such as acquiring primers from published literatures.In recent years,with the increase of EST database,SSRs explored from dbEST have been used in many species.In addition,the conservation of repeat sequence and flanking regions across taxa makes it possible that microsatellite primers from one species can be used to detect polymorphism at homologous sites in related species.The last method is to exploit microsatellite loci from genomic DNA,including the traditional method of genomic library and the novel approach of enriched library such as magnetic bead hybridization and nylon membrane hybridization.

Key words： Microsatellite(SSR) EST-SSRs Interspecific transfer amplification Magnetic bead hybridization Nylon membrane hybridization RAPD

Received: 25 August 2005

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	ZENG Qing-guo
	CHEN Yi-yan

Cite this article:

ZENG Qing-guo,CHEN Yi-yan. The Methods of Isolating Microsatellite Loci[J]. , 2005, 24(4): 368-372.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2005/V24/I4/368

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