黑麂粪便DNA提取及其PCR检测

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Abstract

The fresh faecal and dated faecal of black muntjac(Muntiacus crinifrons) were collected, and their DNA were extracted. For comparing the extraction efficiency, the muscle tissue and skins samples of black muntjac were extracted. The electrophoresis results showed that this method can efficiently extract faecal DNA from black muntjac's faecal samples. This method overcame previous barriers to molecular scatology, new approach is developed for noninvasive samping of black muntjac and chinese sika deer. Noninvasive samping can expand the extension of sampling for genetic population structure and genetic diversity of highly endangered cervid.

Key words： Black muntjac Faecal DNA DNA extraction PCR Noninvasive samping

Received: 25 April 2006

:	Q953
	Q959.843

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	Articles by authors
	CHENG Hong-yi
	BAO Yi-xin
	ZHENG Rong-quan
	CHEN Liang
	GE Bao-ming

Cite this article:

CHENG Hong-yi,BAO Yi-xin,ZHENG Rong-quan, et al. Black Muntjac Faecal DNA Extraction Techniques in PCR Reaction[J]. , 2006, 25(2): 158-161.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2006/V25/I2/158

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