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| Effect of Vitamin-B1,Vitamin-B12 and Vitamin-H on the growth and carotenoid content of Haematococcus pluvialis |
| LI Li-xin, DUAN Shun-shan, LI Ai-fen, XU Ning |
| Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China |
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Abstract An economic microalgae Haematococcus pluvialis CH-1 was used as experimental material. An experiment of adding six grades of concentrations of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively was conducted. Cell density, biomass, chlorophyll-a, and carotenoid content were measured. The results showed that the growth of H. pluvialis was accelerated significantly by adding of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively. The optimal adding concentration of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively for H. pluvialis was 10 mg·L-1, 50μg·L-1, and 500μg·L-1. Under the optimal concentration of Vitamin-B1, Vitamin-B12 and Vitamin-H respectively for H. pluvialis, cells density, biomass, chlorophyll-a and carotenoid content were enhanced with Vitamin-B1:20.1%, 14.6%, 23.2% and 21.3%; Vitamin-B12:29.5%, 30.0%, 28.0% and 24.4%; Vitamin-H:17.1%, 29.2%, 21.8% and 10.1% higher than the control respectively. On the mass culture of motile cells of Haematococcus pluvialis, properly adding Vitamin-B1, Vitamin-B12 and Vitamin-H respectively was effective for increase cells density, biomass, chlorophyll-a and carotenoid content.
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Received: 25 April 2006
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