通入CO2和补加氮源对紫球藻生长和代谢物质积累的影响

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Abstract

The effects of CO₂ concentration and nitrogen source KNO₃ addition on the growth and major metabolic substances accumulation of Porphyridium sp. UTEX 637 were researched by culturing it in the Φ6×60cm cylindrical glass photobioreactor. The results showed that appropriate concentration of CO₂ could significantly promote the algal biomass and the content of exopolysaccharides, water-soluble protein and total lipids. The most suitable CO₂ concentration was 1%. The maximum cell dry weight of experimental group was 8.14 g/L(1%CO₂), which was 4.93 times of the control group. The maximum output of exopolysaccharides was 238.38 mg/L, which was significantly higher than the control group. Adding nitrogen source KNO₃ had apparent stimulative effect on the algal cell growth, exopolysaccharides, and water-soluble protein content. The maximum cell biomass and exopolysaccharides content were 1.5 and 1.25 times of the control group, but it did not contribute to the total lipids accumulation.

Key words： Porphyridium sp. CO2 concentration KNO3 biomass metabolic substances

Received: 25 February 2013

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	Articles by authors
	HAN Juan
	WANG Jiang-hong
	LI Ai-fen
	ZHANG Cheng-wu

Cite this article:

HAN Juan,WANG Jiang-hong,LI Ai-fen, et al. Effect of CO2 concentration and nitrogen source addition on growth and major metabolic substances accumulation in Porphyridium sp.[J]. , 2013, 32(1): 51-56.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I1/51

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