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| Study on the susceptibility tests of antibiotics and obtain axenic culture of Nannochloropsis ocutala |
| LIU Xiao-juan, DUAN Shun-shan |
| Institute of Hydrobiology, Jinan University, Guangzhou 510632, China |
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Abstract Effects of 5 antibiotics(chloramphenicol,penicillin,streptomycin,kanamycin sulfate,gentamycini sulfate) at different concentrations on the growth of Nannochloropsis ocutala were studied.Results showed that at 50 μg·mL-1,growth of N.ocutala was significantly inhibited by chloramphenicol,the highest inhibition was 85.30%,Ic50 of 2 d and 4 d was 293.64 μg·mL-1 and 67.69 μg·mL-1,respectively.Followed by penicillin,gentamycini sulfate,and kanamycin sulfate,the inhibition rate of 4 d was 8.95%,7.62% and 3.84%,respectively.And 2 d Ic50 of gentamycini sulfate was 316.40 μg·mL-1.Growth of N.ocutala was not inhibited significantly by streptomycin,the inhibition rate was only 3%.According to susceptibility tests of antibiotics,a strategy of serial antibiotic treatments to obtain axenic culture was carried out:50 μg·mL-1 penicillin,50 μg·mL-1 gentamycini sulfate,100 μg·mL-1 kanamycin sulphate,50 μg·mL-1 streptomycin.
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Received: 25 December 2006
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