Eco-tourism in Nature reserve can not only improve the public environment awareness and increase the reserve income but also impact on the eco-equilibrium and the fauna and flora resources. How to treat the relationship between the natural conservation and the eco-tourism has already attracted ever-greater concern from more and more people. The authors have surveyed and analyzed the eco-tourism in both national and international nature reserves. The conclusion is that when the reserve carries out eco-tourism the effective management,the measurement should be taken with a scientific plan. A perfect management and monitoring system should be taken as well in order to prevent the nature resources and the eco-environment.