大批量测定植物(互花米草)叶面积的一种新方法

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Abstract

Accurate measurement of leaf area is a foundation for evaluating energy flux, carbon flux, and water using of ecosystem. Current methods for measuring leaf area have various limitations and scope of use. Some of them are easy-to-use and accurate, but can only be used for small batches. It is very difficult for most methods to measure leaf areas in large batches in a relatively short period of time. In this study, we took Spartina alterniflora as an example to discuss a method based on scanner and dry-leaves weight, combined with Photoshop image processing technology, which can measure leaf areas instantly in large batches. Tested by a known power function relation between leaf length (y) and leaf pixel (x), ie. y=419.85x^1.9693 (R≈1.00), our new method only yielded 3.651% (n=30) relative error. There were no significant difference between this method and a classic square method (P=0.473). It is concluded that this method can be used to measure leaf area in large batches in a relatively short period of time, and to measure various shapes of leaves with little change in the vein.

Key words： scanner Spartina alterniflora leaf area pixel leaf area curve

Received: 25 August 2007

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	Articles by authors
	ZHAO Cong-jiao
	DENG Zi-fa
	ZHOU Chang-fang
	CHEN Lin
	LIU Jing-na
	AN Shu-qing
	SHEN Rui-ling

Cite this article:

ZHAO Cong-jiao,DENG Zi-fa,ZHOU Chang-fang, et al. A new method of instantly measuring leaf area in large batches:a case study of Spartina alterniflora[J]. , 2007, 26(4): 327-331.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2007/V26/I4/327

[1] Gower S T,Norman J M.1991.Rapid estimation of leaf area index in conifer and broad-leaf plantations[J].Ecology,72:1896-1900.

[2] Marshll J D,Waring R H.1986.Comparison of methods of estimating leaf-area index in old-growth Douglas-Fir[J].Ecology,67(4):975-979.

[3] 王希群,马履一,贾忠奎,等.2005.叶面积指数的研究和应用进展[J].生态学杂志,24(5):537-541.

[4] Knight D H.1973.Leaf Area dynamics of a shortgrass Prairie in Colorado[J].Ecology,54(4):891-896.

[5] 肖强,叶文景,朱珠,等.2005.利用数码相机和Photoshop软件非破坏性测定叶面积的简便方法[J].生态学杂志,24(6):711-714.

[6] 张健钦,王秀,龚建华,等.2004.基于机器视觉技术的叶面积测量系统实现[J].自然科学进展,14(11):1304-1310.

[7] 袁哲明,傅凌才.1997.用扫描仪测定害虫食叶面积[J].湖南农业大学学报,23(5):472-473.

[8] 冯冬霞,施生锦.2005.叶面积测定方法的研究效果初报[J].中国农学通报,21(6):150-153.

[9] 徐国万,卓荣宗.1985.我国引种互花米草的初步研究[J].南京大学学报(米草研究的进展——22年来的研究成果论文集),212-225.

[10] Daehler C C,Strong D R.1996.Status,prediction and prevention of introducer cordgrass Spartina spp.Invations in pacific estuaries,USA[J].Biological Conservation,78,51-58.

[11] Seliskar D M,Gallagher J L,Burdick D M,et al.2002.The regulation of ecosystem functions by ecotypic variation in the dominant plant:a Spartina alterniflora salt-marsh case study[J].Journal of Ecology,90,1-11.

[12] 陈琳,邓自发,安树青,等.2007.淡咸水轮换浇灌抑制互花米草(Spartina alterniflora Loisel.)的生长和繁殖[J].植物生态学报,31(4):645-651.

[13] Chung C H,Zhou R Z,Xu G W.2004.Creation of Spartina plantations for reclaiming Dongtai,China,tidal flats and offshore sands[J].Ecological Engineering,23,135-150.

[14] 刘永学,李满春,张忍顺.2004.江苏沿海互花米草盐沼动态变化及影响因素研究[J].湿地科学,2(2):116-121.

[15] 邓自发,安树青,智颖飙,等.2006.外来种互花米草入侵模式与爆发机制[J].生态学报,26(8):2678-2686.

[16] Blum M J,Christina M S,Debra R A,et al.2004.Characterization of microsatellite loci in Spartina species(Poaceae)[J].Molecular Ecology Notes,4:39-42.

[17] 王永皎,张引,张三元.2006.基于图像处理的植物叶面积测量方法[J].计算机工程,32(8):210-212.

[18] 杨劲峰,陈清,韩晓日,等2002.数字图像处理技术在蔬菜叶面积测量中的应用[J].农业工程学报,18(4):155-158.