SCGE技术检测镉对背角无齿蚌血细胞DNA的损伤

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Abstract

In this study,mussels(Anodonta woodiana woodiana) were chosen as the research object.We assessed the possible DNA damage in haemocytes of mussels using the single cell gel electrophoresisassay.Results showed haemocytes images which exposed to cadimium presented comet tails,compared the exposed groups with negative control group,existed significant differences.Mussels were exposed to different cadmium chloride concentrations(0,1,10,50 mg·L^-1) respectively for 72 hours,the results showed that the average length of DNA migration and tai lDNA% in all exposed groups was significantly higher than that of negative control group(p<0.01) and existed significant dose-effect relationship.Otherwise,at the concentration of 10 mg·L^-1,four time-effect groups(24,48,72,96 h) and a zero time control group were set up,the average length of DNA migration and tail DNA% under different exposed times in cadmium chloride was significantly higher than zero time group(p<0.01),but no significant time-effect relationship among the tested concentrations.

Key words： cadmium chloride Anodonta woodiana woodiana single cell gel electrophoresis(SCGE) DNA damage comet image

Received: 25 April 2008

Q178.51+7

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	Articles by authors
	ZHOU Kan
	OUYANG Shan
	WU Xiao-ping

Cite this article:

ZHOU Kan,OUYANG Shan,WU Xiao-ping. Detection of DNA Damages in Hemocytesof A. woodiana woodiana Induced by Cadmium with single Cell Gel electrophoresis Assay[J]. , 2008, 27(2): 90-94.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2008/V27/I2/90

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