环境因子及超低温冻存对黄姑鱼精子活力的影响

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Abstract

We investigated the effects of environmental factors and cryopreservation on the vitality of sperm in Nibea albiflora.The results indicated that the suitable salinity for sperm activation and movement was 25-35 while pH value 7.5-8.5.The highest activation rate,the longest moving time and life-span of sperm were(87.00±5.54)%,(336±14.02) s,(405.33±12.22) s,respectively when the sperm was activated in the solutions(salinity25,pH 8.0-8.5).Also the suitable concentration of KCl,NaCl,MgCl₂,glucose solutions for sperm activation was 300-500mmol·L^-1,600mmol·L^-1,800-1000 mmol·L^-1 and 900 mmol·L^-1,respectively.The sperm could not be activated by HCO₃^- free artificial seawater and more than 80% of sperms were activated by Ca²⁺-free,Mg²⁺-free artificial seawater while the moving time and life-span of sperm was shortened significantly.The activation rate and moving time of cryopreservated sperm exceeded 80% and 200s when Cortland and HBSS solutions were used as extender and 10%EG as cryoprotectant.

Key words： Nibea albiflora sperm motility cryopreservation environmental factors

Received: 25 August 2010

S965.325

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	Articles by authors
	YAN Jia-qiang
	WEI Ping
	Jiang Jian-hu
	LI Hai-yan
	ZHU Jun-quan

Cite this article:

YAN Jia-qiang,WEI Ping,Jiang Jian-hu, et al. Effects of environmental factors and cryopreservaytion on the vitality of sperm in Nibea albiflora[J]. , 2010, 29(4): 339-344.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I4/339

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