植物叶片和采后果蔬的过氧化氢酶活性

Abstract
Figure/Table
References
Related Citation (4)

Download: PDF (574 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

In order to understand the physiological properties of catalase(CAT) activity among different plant species,we measured CAT activity and hydrogen peroxide content in 40 plant leaves and 30 postharvest fruits and vegetables.The results show that CAT activity in leaves of C3 plants is higher than that of C4 and CAM plants,and CAT activity in C4 plants is higher than that in CAM plants.Leaf CAT activity reflects the physiological and ecological properties of plants in different photosynthetic metabolic types.CAT activity of leaf vegetables is the highest,followed by flower vegetables,and it is higher in corm leaf vegetables and fruit vegetables than in the bulbous vegetables,subterranean stem vegetables and subterranean root vegetables.CAT activity is closely related to physiological and ecological properties of plant organ.

Key words： plant leaves postharvest fruits and vegetables catalase hydrogen peroxide

Received: 25 December 2010

Q945.79

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LI Zhe
	HUANG Lei

Cite this article:

LI Zhe,HUANG Lei. Catalase activity in plant leaves and postharvest fruits and vegetables[J]. , 2010, 29(6): 573-578.

URL:

http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2010/V29/I6/573

[1] Brennan T,Frenkel C.Involvement of hydrogen peroxide in the regulation of senescence in pear[n.Plant Physiology,1997,59:411-16.
[2] Cheng Y,Song C.Hydrogen peroxide homeostasis and signaling in plant cells[J].Science in China Series C:Life Sciences,2006,49:1-11.
[3] Gichner T DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco cellular and acellular comet assays[J].Mutation Research,2003,535:187-193.
[4] Neill S,Desikan R,Hancock J.Hydrogen peroxide signaling[J].Current Opinion in Plant Biology,2002,5:388-395.
[5] Thannickal V J,Fanburg B L.Reactive oxygen species in cell signaling[J].American Journal of Physiology-Lung Cell Molecular Physiology,2000,279:1005-1028.
[6] de Marco A,Roubelakis-Angelakis K A.The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts[J].Plant Physiology,1996,110:137-145.
[7] Shikanai T,Takeda T,Yamauchi H,Sano S,Tomizawa K,Yokota A,Shigeoka S.Inhibition of ascorbate pemxidase under oxidative stress in tobacco having bacterial catalase in chloroplasts[J].FEBS Letters,1998,428:47-51.
[8] Vandenabeele S,Vanderauwera S,Vuylsteke M,Rombauts S,Langebartels C,Seidlitz H K,Zabau M,Van Montagu M,Inze D,Van Breusegem F.Catalase deficiency drastically affects gene expression induced by high light in Arabidopsis thaliana[J].The Plant Journal,2004,39:45-58.
[9] Engel N,Schmidt M,Lutz C,Feierabnd J.Molecular identification,heterologous expression and properties of light-insensitive plant catalases[J].Plant Cell and Environment,2006,29:593-607.
[10] Luna C M,Pastori G M,Driscoll S,Groten K,Bemard S.Drought controls on H₂O₂ accumulation,catalase(CAT)activity and CAT gene expression in wheat[J].Journal of Experimental Botany,2005,56:417-423.
[11] Leon J,Lawton M A,Raskin I.Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco[J] Plant Physiology,1995,108:1673-1678.
[12] Dat J F,Pellinen R Beeckman T,Van De Cotte B.Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco[J].The Plant Journal,2003,33:621-632.
[13] Reilly K,Han Y,Tohme J,Beeching J R.Isolation and characterisation of a cassava catalase expressed during post-harvest physiological deterioration[J].Biochimica et Biophysica Acta,2001,1518:317-323.
[14] Song X,Zhao F.Research progress on catalase in plants[J].Journal of Anhui Agricultural Sciences,2007,35:9824-9827.
[15] Li J,Matsui S.Effects of chilling on antioxidative enzymes in leaves of Cattleya and Cymbidium[J].Journal of The Japanese Society for Horticultural Science,2001,70:360-365(in Japanese).
[16] Okuda T,Matusda Yamanaka A,Sagisaka S.An abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment[J].Plant Physiology,1991,97:1265-1267.
[17] Guan L M,Scandalios J G Hydrogen-peroxide-mediated catalase gene expression in response to wounding[J].Free Radical Biology & Medicine,2000,28:1182-1190.
[18] 刘大永,王维香.1998.过氧化氢对水稻幼苗中CAT和POD活性的影响团.作物学报,24(3):320-324.
[19] Aasada K.The water-water cycle in chloroplasts:Scavenging of active oxygens and dissipation of excess photons[J].Annual Review Plant Physiology and Plant Molecular Biology,1999,50:601-639.
[20] Wu C,Du X,Wang L,Wag W Zhou Q,Tian X.Effect of 1-methylcyclopropene on postharvest quality of Chinese chive scapes[J].Postharvest Biology and Technology,2009,51:431-433.
[21] Techavuthiporn C,Nakano K,Maezawa S.Prediction of ascorbic acid content in broccoli using a model equation of respiration[J] Postharvest Biology and Technology,2008,47:373-381.
[22] Watada A E,Ko N P,Minott D A.Factors affecting quality of fresh-cut horticultural products[J].Postharvest Biology and Technology,1996,9:115-125.
[23] Downes K,Chope G A,Terry L A.Postharvest application of ethylene and 1-methylcyclopropene either before or after curing affects onion(Allium cepa L.) bulb quality during long term cold storage[J].Postharvest Biology and Technology,2010,55:36-44.
[24] Nourian F,Ramaswamya H S.,Kushalappa A C.Kinetics of quality change associated with potatoes stored at different temperatures[J].LWT-Food Science and Technology,2003,36:49-65.
[25] Salah N,Miller N J,Paganga L,Tijburg L,Bolwll G P,Rice-Evabs C.Polyphenolic flavanols as scavengers of aqueous phase radical sandals,and as chain-breaking antioxidants[J].Archives of Biochemistry and Biophysics,1995,322:339-346.