预处理及保存方法对浮游动物碳、氮稳定同位素比值的影响

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Abstract

The effect of pretreatment.and preservation procedures on carbon and nitrogen stable isotope ratios of zooplankton Phyllodiaptomus tunguidus and Diaphanosoma orghidani transamurensis was studied.The results showed that gut contents in P.tunguidus led to a significant deviation in the values of replicates of both δ^I3C and δ^I5N.Lipid extraction resulted in a significant enrichment in δ^I3C and δ^I3N in P.tunguidus.Methanol and ethanol preserved animals showed higher δ^I3C values,while the frozen and formalin-preserved animals resulted in lower δ^I3C values.δ^I5N of two species was affected slightly,but not significantly,by four different preservations in this study.The results suggest that the variation from different treatments must be considered when making cross-study comparisons.Standard protocols for sample preparation and preservation are needed for the study of foodweb structure via stable isotope analysis.

Key words： stable isotope zooplankton pretreatment preservation

Received: 25 April 2011

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	HAN Long
	LIU Zheng-wen

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HAN Long,LIU Zheng-wen. Effect of pretreatment and preservation procedures on carbon and nitrogen stable isotope ratios of zooplankton[J]. , 2011, 30(2): 195-200.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I2/195

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[1]	. [J]. Ecological Science, 2019, 38(2): 199-205.