珠江口沉积物好氧反硝化细菌的筛选及鉴定

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Abstract

Several aerobic denitrifiers were isolated for the first time from sediments of the Pearl River Estuary,and strain A14-1 with the highest nitrogen removal rate was selected.The strain was identified as Rhodococcus aetherivorar according to physiological and biochemical characteristics and sequence analysis of 16S rDNA.It has a denitrification efficiency up to 26.20 mg·L^-1·h^-1,being able to reduce the nitrate concentration of medium from 157.91 mg·L^-1 to 32.07 mg·L^-1 in 48 h.The aerobic denitrification process showed no accumulation of nitrite,indicating the presence of nitrite reductase,which was verified by the nitrite reductase functional gene nirS through amplification.Strain A14-1 was expected to be used in wastewater nitrogen removal,considering its good adaptability and harmlessness to the environment.

Key words： Rhodococcus aetherivorar aerobic denitrifier 16S rDNA nirS

Received: 25 June 2011

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	DENG Chao
	WANG You-Shao

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DENG Chao,WANG You-Shao. Isolation and identification of aerobic denitrifier from sediments of the Pearl River Estuary[J]. , 2011, 30(3): 321-326.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I3/321

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