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Isolation and identification of aerobic denitrifier from sediments of the Pearl River Estuary
DENG Chao1,2,3, WANG You-Shao1,2
1. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; 2. Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen 518121, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
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Abstract  

Several aerobic denitrifiers were isolated for the first time from sediments of the Pearl River Estuary,and strain A14-1 with the highest nitrogen removal rate was selected.The strain was identified as Rhodococcus aetherivorar according to physiological and biochemical characteristics and sequence analysis of 16S rDNA.It has a denitrification efficiency up to 26.20 mg·L-1·h-1,being able to reduce the nitrate concentration of medium from 157.91 mg·L-1 to 32.07 mg·L-1 in 48 h.The aerobic denitrification process showed no accumulation of nitrite,indicating the presence of nitrite reductase,which was verified by the nitrite reductase functional gene nirS through amplification.Strain A14-1 was expected to be used in wastewater nitrogen removal,considering its good adaptability and harmlessness to the environment.

Key wordsRhodococcus aetherivorar      aerobic denitrifier      16S rDNA      nirS     
Received: 25 June 2011     
:  Q93  
  Q14  
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DENG Chao
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Cite this article:   
DENG Chao,WANG You-Shao. Isolation and identification of aerobic denitrifier from sediments of the Pearl River Estuary[J]. , 2011, 30(3): 321-326.
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