环境水体中肠道感染菌群与几种典型致病菌的QPCR检测及相关性分析

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Abstract

The copies of enteric infectious flora and three typical pathogenic bacteria in surface waters were detected continuously for four months by using quantitative PCR(QPCR) method.The results show that in the seriously polluted waters,E.coli is dominant among the enteric infectious flora,and the copies of E.coli are almost the same as enteric infectious flora detected by universal primers.When the copies of enteric infectious flora in water are more than 10⁴ copies·100mL^-1,S.typhrmurium and Shigella flexneri can be detected continuously,which can be considered as one of the indicators of water pathogenic pollution.In the clean and slightly polluted waters,when the copies of enteric infectious flora are less than 10⁴ copies·100mL^-1,the enteric pathogens are mainly other bacteria except the three kinds of typical pathogenic bacteria mentioned above.The copies and detection frequency of the three kinds bacteria have no significant correlation with the enteric infectious flora monitored by universal primers.

Key words： surface water enteric infectious flora pathogenic bacteria quantitative PCR(QPCR) correlation

Received: 25 August 2011

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	Articles by authors
	ZHANG Ai-ning
	LIU Li
	SHI Yan-dan
	LIU Yong-jun

Cite this article:

ZHANG Ai-ning,LIU Li,SHI Yan-dan, et al. Detection and correlativity analysis of enteric infectious flora and three typical pathogenic bacteria in surface waters by QPCR[J]. , 2011, 30(4): 426-430.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2011/V30/I4/426

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