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Identification and phylogenetic analysis of culturable bacteria in Daya Bay
JIANG Xiao-liang, WANG Zhao-hui*, WANG Jian, XIONG Yi-jun, LIN Lang-cong
Institute of Hydrobiology, Jinan University, Guangzhou 510632, China
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Abstract  

Culturable bacteria were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and Intrasporangium, which were rarely reported, were also isolated. The value of Shannon-Wiener`s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.

Key wordsDaya Bay      culturable bacteria      16S rRNA gene      phylogenetic analysis     
Received: 25 June 2013     
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JIANG Xiao-liang
WANG Zhao-hui
WANG Jian
XIONG Yi-jun
LIN Lang-cong
Cite this article:   
JIANG Xiao-liang,WANG Zhao-hui,WANG Jian, et al. Identification and phylogenetic analysis of culturable bacteria in Daya Bay[J]. , 2013, 32(3): 290-297.
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http://www.ecolsci.com/EN/     OR     http://www.ecolsci.com/EN/Y2013/V32/I3/290
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