大亚湾海域可培养细菌鉴定及系统发育分析

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Abstract

Culturable bacteria were isolated from surface seawater in Daya Bay, and identified by means of 16S rRNA gene V3 region sequences. A phylogenetic tree was constructed using 35 sequences in this study and 46 sequences from GenBank. Seventy bacterial isolates were isolated and cultured from surface water of 9 stations in Daya Bay, which were classified into 35 categories. Within them, 40% belonged to Alphaproteobacteria, 35.7% to Actinobacteria, and 1.4% to Betaproteobacteria. Meanwhile, two genera, Kytococcus and Intrasporangium, which were rarely reported, were also isolated. The value of Shannon-Wiener`s index was 3.21, indicating a diversity of bacteria in Daya Bay. The high diversity of the culturable bacteria and abundance of Alphaproteobacteria suggested the good water quality and the potential microbial resources in Daya Bay.

Key words： Daya Bay culturable bacteria 16S rRNA gene phylogenetic analysis

Received: 25 June 2013

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	Articles by authors
	JIANG Xiao-liang
	WANG Zhao-hui
	WANG Jian
	XIONG Yi-jun
	LIN Lang-cong

Cite this article:

JIANG Xiao-liang,WANG Zhao-hui,WANG Jian, et al. Identification and phylogenetic analysis of culturable bacteria in Daya Bay[J]. , 2013, 32(3): 290-297.

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http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2013/V32/I3/290

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