人工湿地不同植物根际微生物群落多样性比较研究

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2275 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

Rhizosphere microbial community diversity of different plants Phragmites australis, Typha latifolia, and Lolium perenne grown in constructed wetland was studied by plate count methods and PCR-DGGE. The results were as followed.Rhizosphere of different plants was different in community structure and microbial quantity. The microbial quantities in rhizosphere zone were significantly different from those in non-rhizosphere zone (P<0.05). Moreover, there were different community structure and dominant populations between rhizosphere and non-rhizosphere. Phragmites australis rhizosphere had the largest number of common species and species evenness. At the same time, it had the most significant rhizosphereeffect (R/S).The biggest different diversity index between rhizosphere and non-rhizosphere was also in P. australis, which showed that it could better influence the enrichment and distribution of soil microbes, and improve the microbial communitiy diversity.

Key words： constructed wetland rhizosphere community diversity PCR-DGGE

Received: 25 June 2012

X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WU Yu-kun
	LIU Yong-Jun
	SI Ying-ming
	XIONG Jia-qing
	WANG Xiao-chang

Cite this article:

WU Yu-kun,LIU Yong-Jun,SI Ying-ming, et al. Study on rhizosphere microbial community diversity of different plants grownin the constructed wetland[J]. , 2012, 31(3): 318-323.

URL:

http://www.ecolsci.com/EN/ OR http://www.ecolsci.com/EN/Y2012/V31/I3/318

[1] 吕炳南,董春娟.2007.污水好氧处理新工艺[M].哈尔滨:哈尔滨工业大学出版社.
[2] 李海燕,王金成,梁健.辽东栎与油松根际土壤微生物群落结构比较研究[J].土壤通报,2010,41(6):1360-1364.
[3] 项学敏,宋春霞,李彦生,孙翔宇.湿地植物芦苇和香蒲根际微生物特性研究[J].环境保护科学,2004,30(124):35-38.
[4] 奉小忧,宋永会,曾清如,彭剑峰,刘雪瑜,Rhee JS,Lamchaturapatr J.不同植物人工湿地净化效果及基质微生物状况差异分析[J].环境科学研究,2011,24(9):1035-1041.
[5] 黄德峰,李田,陆斌.复合垂直流人工湿地污染物去除及微生物群落结构的PCR-DGGE分析[J].环境科学研究,2007,20(6):137-141.
[6] Gelsomino A,keijzer-Wolters A C,Cacco G,van ElsasJ D.Assessment of bacterial community structure insoil by polymerase chain reaction and denaturinggradient gel electrophoresis[J].J Microbiol Methods,1999,38(1-2):1-15.
[7] Yang C H,Crowley D E,Menge J A.16S rDNAfingerprinting of rhizosphere bacterial communitiesassociated with healthy and Phytophthora infectedavocado roots[J].FEMS Microbiol Ecol,2001,35(2):129-136.
[8] Muyzer G,de Waal E C,Uitterlinden A G.Profiling ofcomplex microbial population by denaturing gradientgel electrophoresis analysis of polymerase chainreaction-amplified genes coding for 16S rRNA[J].Appl Environ Microbiol,1993,59(3):695-700.
[9] Ibekwe A M,Papiernik S K,Gan J,Yates S R,Yang CH,Crowley D E.Impact of fumigants on soilmicrobial communities[J].Appl Environ Microbiol,2001,67(7):3245-3257.
[10] Kozdrój J,van Elsas J D.Structural diversity ofmicrobial communities in arable soils of a heavilyindustrialized area determined by PCR-DGGEfingerprinting and FAME profiling[J].Appl Soil Ecol,2001,17(1):31-42.
[11] 李振高,骆永明,滕应.2008.土壤与环境微生物研究法[M].北京:科学出版社.
[12] 徐光辉,郑洪元.1986.土壤微生物分析方法手册[M].北京:农业出版社.
[13] 赵祥伟,骆永明,滕应,李振高,宋静,吴龙华.重金属复合污染农田土壤的微生物群落遗传多样性研究[J].环境科学学报,2005,25(2):186-191.
[14] 张斌,孙宝盛,季民,赵祖国.MBR中微生物群落结构的演变与分析[J].环境科学学报,2008,28(11):2192-2199.
[15] 刘慧杰,杨彩云,田藴,林光辉,郑天凌.基于PCR-DGGE技术的红树林区微生物群落结构[J].微生物学报,2010,50(7):923-930.
[16] 尤朝阳,万玉龙,刘汤勋.黄菖蒲人工湿地微生物分布特点研究[J].安徽农业科学,2010,38(35):20233-20235.
[17] 申建波,张福锁.根分泌物的生态效应[J].中国农业科技导报,1999,1(4):21-27.
[18] 刘开朗,王加启,卜登攀,李旦,于萍,赵圣国.环境微生物群落结构与功能多样性研究方法[J].生态学报,2010,30(4):1074-1080.
[19] 王青璐,彭明春,赵安娜,黄丽,刘韬,梁国军,刘路明.人工湿地中影响微生物空间分布因素的探讨[J].环境科学导刊,2009,28(2):1-3.