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The characteristics of pigment-protein complexes from brown alga Undaria pinnatifida |
Li Aifen1, Hu Ren1, Liu Ningning1, Gong Baoan2, Zhou Baicheng3 |
1. Institute of Hydrobiology, Jinan University, Guangzhou 510632; 2. College of Chemistry and biology, Yantai University, Yantai 264005; 3. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 |
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Abstract When the thylakoid membranes of brown alga Undaria pinnatifida were solubilized with DMG and subjected to PAGE, eight pigment-protein complexes were resolved.Both CP Ⅰ a and CP Ⅰ contain a polypeptide of 66 kDa,and they have a long- wavelength fluorescence emission peak at 715 nm at 77 K,the excitation spectra shows CP Ⅰ a is fucoxanthin-chla/c-protein complex and CP Ⅰ is chla-protein complex.CPa contains 51,37,34 and 20 kDa polypeptides,its fluorescence emission maximum at 77 K is at 683 nm,the excitation spectra shows that CPa is a fucoxanthin-chla/c-protein complex.The other five complexes are related to LHC,they all present a major polypeptide component of 20 kDa,the spectra characteristics of LHC1 and LHC3 are similar,they are all fucoxanthin-chla/c-protein complexes,LHC2,LHC4 and LHC5 are chla/c-protein complexes.
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Received: 25 June 2002
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